摘要
目的:从细胞生物学水平观察二仙汤及其拆方和部分提取物对原代培养的雌性大鼠垂体前叶细胞的作用。方法:在原代培养的大鼠垂体前叶细胞中直接加入二仙汤全方或其拆方及部分提取物,用放射免疫法测定培养液中黄体生成素(luteinizing hormone,LH)和卵泡刺激素(follicle-stimulating hormone,FSH)的含量。结果:二仙汤全方及其拆方滋阴泻火方和调理冲任方直接加药能促进大鼠垂体前叶细胞LH和FSH的分泌;温肾益精方也有一定促进趋势。二甲亚砜作为淫羊藿苷和仙茅苷的溶剂对实验结果有一定影响。结论:二仙汤全方及其拆方滋阴泻火方和调理冲任方对垂体前叶细胞LH和FSH的分泌有促进作用。
Objective： To investigate the effects of Erxian Decoction （EXD）, a traditional Chinese compound herbal medicine and its separate prescriptions such as Wenshen Yijing Recipe （WSYJR, a recipe for warming kidney and replenishing essence）, Ziyin Xiehuo Recipe （ZYXHR, a recipe for nourishing yin and dispersing fire） and Tiaoli Chongren Recipe （TLCRR, a recipe for regulating thoroughfare and conception vessels） and some extracts from EXD on the levels of LH and FSH in the primary cultured anterior pituitary cells from female rats. Methods： EXD, its separate prescriptions and traditional Chinese herbal extracts from EXD were added directly to the incubators. The levels of LH and FSH were tested by radioimmunoassay. Results： The levels of LH and FSH in the supernatant of anterior pituitary cells treated by EXD or its separate prescriptions including ZYXHR and TLCRR were increased significantly as compared with those in the blank control. There was a tendency for stimulating the secretions of LH and FSH in the WSYJR-treated group too. Dimethyl sulfoxide as the solvent of icariin and curculigoside could interfere with the results. Conclusion： EXD and its separate prescriptions such as ZYXHR and TLCRR can increase the levels of LH and FSH significantly.
出处
《中西医结合学报》
CAS
2007年第6期665-669,共5页
Journal of Chinese Integrative Medicine
基金
教育部优秀青年教师资助计划项目(No.1632)
关键词
垂体
前叶
黄体生成素
促卵泡激素
大鼠
pituitary gland, anterior
luteinizing hormone
follicle-stimulating hormone
rats
