摘要
目的:探讨Toll样受体-2(TLR-2)在慢性阻塞性肺疾病(COPD)诱导痰巨噬细胞上的表达及其与气道炎症的关系。方法:20例COPD急性发作期(AECOPD)、16例COPD稳定期患者、年龄相当的正常人、支气管肺炎患者及健肺支气管肺泡灌洗者各10例纳入本研究。3%-5%氯化钠注射液进行痰液诱导,瑞氏染色行痰细胞分类计数,人淋巴细胞分离液行诱导痰单个核细胞分离。荧光物标记的抗人Toll样受体-2及CD14抗体行巨噬细胞双标,用流式细胞仪检测巨噬细胞平均荧光强度(rMFI)。结果:AECOPD、COPD稳定期、肺炎组诱导痰细胞总数、巨噬细胞及中性粒细胞数均显著高于正常对照组(P<0.01),肺炎组治疗后中性粒细胞数显著低于COPD稳定期组(P<0.01)。正常对照组及肺炎组疗前巨噬细胞TLR-2表达水平分别显著高于COPD稳定期、AECOPD治疗前后(P<0.01),肺炎组疗后则接近正常(P>0.05)。COPD稳定期、AECOPD巨噬细胞TLR-2表达水平与气道中性粒细胞数呈显著正相关(r=0.572,0.892,0.649,P<0.05或0.01)。结论:COPD诱导痰巨噬细胞TLR-2表达水平较正常人低下,这与COPD慢性气道炎症的形成密切相关。
Objective： To explore the expression of Toll-like receptor 2 on macrophages of induced sputum in chronic obstructive pulmonary disease （COPD）. Methods. Twenty patients with acute exacerbation of COPD（AECOPD）, 16 patients with stable COPD, and 10 age-matched normal people and 10 bronchial pneumonia patients by bronchial-alveolar lavage were enrolled in the study. Induced sputum were collected by inhalation of 3 %-5 % sodium chloride. Cell numbers and differentials in sputum cell suspension were counted with Wright＇s stain. Mononuclear cells in induced sputum were separated by Ficoll-Hypaque. Macrophages of induced sputum were labelled by FITC-anti Toll-Like receptor 2（TCR-2） antibody and PE anti CD14 antibody, and flow cytometry were used to analyze relative mean fluorescence intensity（rMFI） of labelled macrophages. Resuits： The number of total sputum cells, macrophages and neutrophils in stable COPD, AECOPD and pneumonia group were significantly higher than those in normal group （P〈0.01）. In pneumonia group neutrophils counts were significantly less than in stable COPD group （P〈0.01） after treatment. The level of TLR-2 expression in normal group was not different from that in bronchial-alveolar lavage （P〉0.05）, but was significantly higher than in stable COPD （P〈 0.01）. In AECOPD, TLR-2 level was significantly higher than in stable COPD （P〈0.01） before treatment and kept at relative higher level after treatment （P〈0.01）, but was significantly lower than in pneumonia group （P〈0.01）, which decreased to normal after treatment （P〉0.05）. The level of TLR-2 expression in stable COPD, AECOPD （before and after treatment） or pneumonia group was significantly positively correlated with neutrophils （r = 0. 572, 0. 892, 0. 649 and 0. 788 respectively, all P（0.05 or 0. 01）. Conclusion： TLR-2 expression decreases on macrophages of induced sputum in COPD, which might result in persistent airway inflammation.
出处
《武汉大学学报（医学版）》
CAS
2007年第6期770-773,共4页
Medical Journal of Wuhan University
