摘要
Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method.
Gl2^e is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gl2^e by using the F2 segregating population containing 1599 plants derived from the cross of G hirsutum genetic standard line TM-1 and G barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gl2^e was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum x G. barbadense BC1 published by our laboratory, Gl2^e was Iocared between CIR362 and NAU2251b, NAU3860b, STV033, with a genetic distance 9.27 and 0.96 cM, respectively. This result is useful for cloning Gl2^e gene by map-based cloning method.
基金
Supported by the National Basic Research Program of China (Grant No. 2007CB108805)
Program for Changjiang Scholars and Innovative Re-search Team in University (Grant No. IRT0432)
关键词
显性
突变基因
棉花
种子
cotton
Gl^e_2 gene
glandless frait
molecular marker
fine mapping
