摘要
观察不同浓度的三氧化二砷（Ａｓ２Ｏ３）对急性早幼粒细胞性白血病（ＡＰＬ）细胞株ＮＢ４细胞的影响。方法不同浓度的三氧化二砷（０．１～２μｍｏｌ／Ｌ）与ＡＰＬ细胞株ＮＢ４细胞共同孵育一定时间后，观察ＮＢ４细胞的存活，细胞形态学，免疫表型（ＣＤ１１ｂ，ＣＤ３３），细胞ＤＮＡ含量分布及ＰＭＬ／ＰＭＬ－ＲＡＲα蛋白在细胞内定位的改变。结果经１～２μｍｏｌ／Ｌ氧化砷处理的ＮＢ４细胞呈现凋亡的特征性改变。在细胞形态学上表现为胞膜完整，染色质固缩及核碎裂。流式细胞仪分析显示与三氧化二砷作用浓度及时间相关的亚Ｇ１期细胞。经０．１～０．２５μｍｏｌ／Ｌ三氧化二砷处理１０天的ＮＢ４细胞呈现某些与分化相关的形态学改变和分化相关抗原（ＣＤ１１ｂ和ＣＤ３３）的调变，而０．１～２μｍｏｌ／Ｌ的三氧化二砷均能快速调变和减少ＰＭＬ／ＰＭＬ－ＲＡＲα蛋白的表达。结论氧化砷对ＮＢ４细胞具有诱导凋亡和不完全分化双重作用，并能快速调变及降解ＰＭＬ／ＰＭＬ－ＲＡＲα蛋白的表达。
Objective To investigate the effects of a wide range of concentrations of As 2O 3 on NB 4 cells. Methods Cell morphology, cell DNA content distribution, CD11b and CD33 antigens and nitroblue tetrazolium (NBT) reductions were evaluated in an APL cell line NB 4 cells with or without As 2O 3 treatment. In addition, immunofluorescent analysis for APL marker molecule PML RAR α was also performed. Results 1～2 μmol/L of As 2O 3 treated NB 4 cells presented morphologically some features of apoptotic cells such as intact cell membrane, chromatin condensation and nuclear fragmentation. Sub G1 cells ,whose percentage presents concentration and time dependency, were observed by flow cytometer. Otherwise, NB 4 cells with the treatment of As 2O 3 at 0.1～0.25 μmol/L for a long time (10 days) have differentiation related morphology, and their differentiation antigens CD11b and CD33 were also modulated to some extent. In addition, 0.1～2 μmol/L of As 2O 3 could rapidly and effectively modulate and degradate PML/PML RAR α proteins. Conclusion As 2O 3 had double effects (induction of apoptosis and imcomplete differentiation) on NB 4 cells, which could associate with rapid modulation and degradation of PML/PML RARα proteins.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第7期509-512,共4页
National Medical Journal of China
基金
国家自然科学基金
卫生部优秀青年基金
上海科技启明星计划
上海血液学研究所胡应洲基金
关键词
氧化砷
白血病
早幼粒细胞性
急性
细胞分化
Arsenic trioxide Leukemia, myelocytic, acute Cell differentiation
