摘要
目的:研究重组人血管内皮生长因子(VEGF)基因对游离皮瓣、皮片及游离颗粒脂肪等移植物血管形成的作用。方法:将带有PCD-VEGF165的大肠杆菌接种到LB培养基中,通过碱分裂法制备PCD-VEGF165质粒,再用反蒸发法将质粒包埋于脂质体中。将108只雄性SD大鼠均分为三组,每组36只,每组再分三小组,分别为PCD-VEGF165目的基因组、PCD空白质粒组和生理盐水组,注入后在不同时间点取标本做成石蜡块行常规染色以观察血管生长情况。结果:三种移植组织中目的基因组微血管密度均显著高于其他两对照组(P<0.01),血管直径明显小于对照组(P<0.05),空白质粒组及生理盐水组差异无显著性意义(P>0.05)。结论:VEGF基因生物活性稳定,特异性强,持续时间长。注射转VEGF基因的质粒后可诱导移植组织周围新血管的形成,增加血流灌注。
Objective To investigate the angiogenesis of rhVEGF in the free transplanted tissues,such as flaps,grafts and free autologous pearl fat grafts in rats.Methods The plasmids with PCD-VEGF165 were made by alkalielysis method after the E.coli with PCD-VEGF165 was cultivated in the LB culture medium,and then the plasmid were buried in the liposome by reverse evaporation method.One hundred and eight Sprague-Dawley rats were divided into three groups：the flaps,the grafts and the free fat.There were thirty six animals in each groups.And then all the groups were divided into three secondary groups：Injected with the plasmid cDNA encoding the 165-amino acid isoform of VEGF as the experiment group,the blank plasmid DNA as the negative control group and the normal saline as the frank control group.After injection the specimens were cut and harvested for pathological test.Results The microvessel count in the PCD-VEGF165 groups were significantly higher than the other two control groups（P〈0.01）.And the average vessel diameter of the PCD-VEGF group was finer than the other two control groups（P〈0.05）.There were no significant difference in the blank PCD groups and the normal saline groups（P〉0.05）.Conclusion The VEGF gene is stable,special and enduring.And the angiogenesis and blood supply increased after it was injected in the free transplanted tissues.
出处
《中国美容医学》
CAS
2007年第10期1325-1328,共4页
Chinese Journal of Aesthetic Medicine
