摘要
目的研究50 Hz工频磁场对离体原代培养的人早孕绒毛滋养细胞分泌功能的影响,探索工频磁场生殖健康效应的可能机制。方法体外分离、培养人早孕绒毛滋养细胞,以0.2、0.4 mT强度的工频磁场分别辐照处理6、12、24、48以及72h,每一辐照组均设立相应时间的平行对照组,用电化学发光免疫分析法测定每组细胞培养液中人绒毛膜促性腺激素(HCG)和黄体酮的含量并对结果进行统计分析。结果0.2 mT工频磁场辐照72 h内,滋养细胞分泌的HCG和黄体酮与空白对照组的差异无统计学意义(P>0.05)。0.4 mT工频磁场辐照48 h内不影响滋养细胞的分泌;辐照72 h,则可以明显抑制滋养细胞分泌HCG与黄体酮(与空白对照组相比,P<0.05)。结论一定强度的工频磁场长时间辐照可抑制绒毛滋养细胞的分泌功能,阈强度可能在0.2至0.4 mT之间。
Objective To explore the possible effects of 50 Hz magnetic fields （MF） exposure on HCG and progesterone secretion of human villous trophoblasts in vitro. Methods The trophoblasts were isolated from human villus by trypsin digestion and incubated in DMEM medium. Then the trophoblasts were exposed to 0.2 mT, 0.4 mT 50 Hz MF for 6 h, 12 h, 24 h, 48 h and 72 h, respectively. Each exposure group was matched to one control group which was from the same villus and cultured with the same condition except the 50 Hz MF exposure. The concentration of human chorionic gonadotropin （HCG） and progesterone in the culture medium was detected by electrochemiluminescence immunoassay. Statistical significance of differences between means was determined by one way-ANOVA with P〈0.05 considered significant. Results Exposure of trophoblasts to 50 Hz MF at 0.2 mT intensity within 72 h did not affect the secretion level of HCG and progesterone （ compared with blank control, P〉0.05）. There was also no significant change of the secretion level of HCG and progesterone when trophoblasts were exposed to 0.4 mT 50 Hz MF within 48 h （compared with blank control,P〉0.05 ）. However, 50 Hz MF inhibited the HCG and progesterone secretion significantly with exposure for 72 h （compared with blank control, P〈0.05）, Conclusion The exposure to 50 Hz MF for long period could inhibit trophoblasts excreting the HCG and progesterone, and the threshold intensity may be between 0.2mT and 0.4 mT.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第10期577-580,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金项目(30470428)
浙江省科技厅项目(2006E10024)
关键词
工频磁场
滋养细胞
人绒毛膜促性腺激素(HCG)
黄体酮
50 Hz magnetic field
Human villous trophoblasts
Human chorionic gonadotropin（HCG）
Progesterone
