摘要
目的:探讨在博莱霉素(BLM)致小鼠肺间质纤维化(IPF)病理过程中,核因子κB(NF-κB)在肺组织中表达的动态变化及其分布。方法:实验组小鼠35只气管内注射BLM,对照组20只注射生理盐水。对照组小鼠于12h,1,14d,实验组于12h,1,3,7,14,28d处死,右肺行支气管肺泡灌洗(BAL)3次,灌洗细胞行p65免疫组化染色,图像分析,左肺病理切片亦行p65免疫组化染色。另分别取两组小鼠各5只,3d后处死,取肺组织原代培养细胞行p65免疫组化染色,图像分析。结果:在BLM诱导的IPF病理过程中,NF-κB在肺间质细胞、肺泡上皮细胞、肺泡内及肺间质内免疫细胞中广泛表达,病程早期较强,随着病程的进展表达强度减弱。实验组BAL细胞核p65表达较对照组明显增强;气管内灌入BLM后1d,p65表达强度明显高于12h、3,7,14,28d;实验组肺组织培养细胞p65平均积分光密度(aiOD)值为462.04±152.25,对照组为1.03±0.37(P<0.05)。结论:NF-κB在IPF病理的早期阶段,可能具有更重要的作用。NF-κB不仅在BAL细胞中,而且在肺间质细胞中的表达明显增强,可能在IPF的病理过程中具有重要作用。
Objective： To investigate the dynamic expression and distribution of nuclear factor-kappa B （NF-κB） in the lung tissues of mice with bleomycin-induced interstitial pulmonary fibrosis （IPF）. Methods： C57BL/6 mice were randomly assigned to receive intratracheal instillation of bleomycin-A5 （BLM-A5） in experiment group and normal saline in control group. Five mice of each group were killed at the following time points： on days 0.5,1 ,and 14 after the intratracheal instillation in control group and on days 0.5,1,3,7,14,and 28 after the intratmcheal instillation in experiment group. Bronchoalveolar lavage （BAL） was performed 3 times in the right lungs,and p65 immunohistochemical staining and image analysis were performed in the lavage cells, p65 immunohistochemical staining was also performed in the pathological sections of the left lungs. Another 5 mice from each group were killed 3 days after the intratracheal instillation, and the primary culture of lung tissues was performed, p65 immunohistochemical staining and image analysis were performed in the cultured cells, and the expression of p65 was expressed as the average integral optical density （aiOD）. Results： NF-κB was generally expressed in the interstitial cells, alveolar epithelial cells, and alveolar and interstitial immunocytes, and the expression was stronger in the early stage of IPF and decreased with the prolongation of the disease course. No expression of NF-κB was found in the tracheal epithelial cells. The expression of p65 in the nuclei of BAL cells in experiment group was significantly higher than that in control group,and the expression of p65 1 day after the intratracheal instillation of bleomycin was significantly higher than that after 0.5,3,7,14, and 28 days. The aiODs of the cultured cells were 462.04±152.25 in experiment group and 1.03±0.37 in control group （P 〈 0.05 ）. The expression of p65 in experiment group was significantly higher than that in control group. Conclusion： NF-κB may play an improtant role in the early stage of IPF. The enhanced expression of NF-κB occurrs not only in BAL cells but also in lung interstitial cells, and the enhanced expression of NF-κB in lung interstitial cells may be involved in bleomycin-induced pulmonary fibrosis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第5期505-507,510,共4页
Journal of China Medical University
基金
国家自然科学基金资助项目(30100076
30570812)
辽宁省自然科学基金资助项目(20042085)
关键词
核因子ΚB
博莱霉素
肺间质纤维化
nuclear factor-kappa B
bleomycin
interstitial pulmonary fibrosis
