摘要
目的观察通络化纤颗粒对博来霉索所致肺纤维化大鼠核因子-κB(NF-κB)、α-平滑肌肌动蛋白(α-SMA)含量的影响。方法将60只健康Wistar大鼠(雌雄兼用)随机分为5组,即空白组、模型组、阳性药(醋酸泼尼松)对照组和通络化纤颗粒高、中剂量组。除空白组外,其余4组用博来霉素注入大鼠气管内制作肺纤维化模型,于造模后第1天起,各组给予相应的药物,分别于第7、28天处死大鼠,取肺组织进行HE染色,光镜下观察大鼠肺组织病理学变化,并进行免疫组化染色,观察大鼠肺组织NF-κB、α-SMA表达的变化。结果在造模后第7天和第28天,空白组无NF-κB及α-SMA阳性表达,模型组有阳性表达,通络化纤颗粒组和阳性药对照组NF-κB、α-SMA的表达较模型组明显降低(P<0.01);且通络化纤颗粒高、中剂量组较阳性药对照组有所降低(P<0.05)。病理学观察显示,模型组炎性反应明显,纤维化程度重;阳性药对照组及通络化纤颗粒高、中剂量组显著减轻。结论通络化纤颗粒可以有效地抑制实验性肺纤维化大鼠NF-κB、α-SMA的表达。
Objective To investigate the effect of Tongluohuaxiankeli（TLHXKL） on NF-κB andα-SMA in rats with pulmonary fibrosis.Methods Sixty healthy Wistar rats were randomly divided into 5 groups,normal group, model group,positive control group and two TLHXKL groups which were treated with high and moderate dose of TLHXKL respectively.Except those rats in normal group,all rats were established pulmonary fibrosis by intra tracheal administration of bleomycin.Different medicines were administrated in different groups from the 1 st day after the models were established.Rats were sacrificed in batches at 2 time points of the 7th and 28th day for observing the pathological changes of lung under light microscope with HE staining and identifying NF-κB andα-SMA in lung tissue by immunohistochemical stain and image quantitative analysis.Results 7 th and 28 th day after model was established, positive expression of NF-κB andα-SMA was not found in normal group,but was found in model group.The NF-κB andα-SMA content in pulmonary tissue of the TLHXKL group and the positive control were remarkably decreased than those of model group in every stage（P〈0.01）.For the TLHXKL group,each dose group could decrease the content of NF-κB andα-SMA in lung tissue,however,the effect of high dose group were more effective.The effect of TLHXKL was superior to that of positive control in every stage（P〈0.05）.Pathological observation showed that inflammatory reaction was obvious in model group,and the fibrosis degree was severe,while the changes were significantly attenuated in positive control guoup and TLHXKL group.Conclusion TLHXKL can effectively reduce the NF-κB andα-SMA content in pulmonary fibrosis rats.
出处
《疑难病杂志》
CAS
2011年第2期126-128,F0003,共4页
Chinese Journal of Difficult and Complicated Cases
