摘要
目的:探讨持续吸入高浓度氧的新生大鼠的生存率、体质量以及肺病理形态学的动态变化。方法:足月新生大鼠,生后分别于90%～95%氧(高氧组)和正常空气中持续暴露(空气组),于1、3、7、14、21d,对比观察其生存情况、称体质量;取肺组织进行HE染色,辐射状肺泡计数(RAC);Masson染色和Gomori银染色,图像定量分析,计算胶原及网状纤维阳性面积百分比。结果:高氧暴露第3天开始,生存率降低,体质量下降,持续至21d;病理观察高氧组早期炎症反应,7d出现肺泡发育阻滞,最终纤维化;RAC数值空气组随着日龄而增加,高氧暴露7、14、21d时较空气组降低(P<0.05或P<0.01)。7d后高氧组的胶原纤维和网状纤维在肺间隔和间质中沉积,阳性面积百分比高于对照组(P<0.05或P<0.01)。结论:持续吸入高浓度氧,使新生大鼠的肺发育受阻,并逐渐出现伴有胶原增加的纤维化改变,与早产儿慢性肺疾病的临床和病理特点相似。
Objective： To determine the dynamic effect of prolonged hyperoxia on survival rate, weight and morphologic characteristics in neonatal rat. Methods： Full-term newborn rats were continuously exposed to oxygen （90%-95% 02） or room air after birth. Survival rate and weight were observed on days 1, 3, 7, 14 and 21 in hyperoxia groups and air controls. Lung histological study for hematoxylin eosin staining and radical alveolar counts （RAC） were performed. Using Masson and Gomori staining and imaging analysis, the areas of eollagenic fibres and reticular fibres were measured. Results： Compared with air controls, the survival rates and weight decreased after hyperoxia exposure 3 days, and lasted to 21d ays. Inflammation response was seen in early stage, the arrest of lung development was evident after 7 days of oxygen exposure, and finally it was interstitial fibrosis. RAC increased with ages, while after 7d ays＇of oxygen exposure, RAC decreased at 7, 14 and 21 days（P 〈 0.05 or P 〈 0.01） compared with air group. After 7 days of oxygen exposure, collagenic fibres and reticular fibres were strongly stained in alveolar septum and interstitum, the areas were larger than that in air group （P 〈 0.05）, which increased significantly with days of oxygen exposure （P 〈 0.01）. Conclusion： With prolonged hyperoxia exposure, lung development in neonatal rats was arrested, and lung fibrosis with the increase of collagen was evident, which is similar to the clinical and pathological changes in CLD of prematurity.
出处
《天津医药》
CAS
北大核心
2007年第5期354-356,I0005,共4页
Tianjin Medical Journal
基金
辽宁省自然科学基金资助项目(项目编号:20022071)
