摘要
目的探讨精神分裂症的外周神经免疫机制及其与临床症状的关系。方法检测精神分裂症患者致炎性细胞因子白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)以及酪氨酸羟化酶(TH)的 mRNA 表达水平,采用逆转录-聚合酶链反应及半定量检测技术,分别检测39例精神分裂症患者(患者组)、25例同胞(同胞组)及30名正常对照(对照组)外周血单个核细胞(PBMC)IL-1β、TNF-α及 TH 基因表达水平,同时应用阳性和阴性症状量表(PANSS)评定精神分裂症患者临床症状。结果患者组、同胞组及对照组 IL-1β的 mRNA 表达水平分别为1.52±1.01、1.52±1.09和0.74±0.38;TNF-α的 mRNA 表达水平分别为1.18±0.99、1.01±0.87和0.70±0.29;TH 的 mRNA 表达水平分别为0.55±0.33、0.61±0.32和0.28±0.20。患者组和同胞组的 IL-1β、TNF-α、TH 的 mRNA 表达水平均明显高于对照组(P<0.05或 P<<0.01)。患者组 IL-1β(r=0.420)、TNF-α(r=0.430)的 mRNA 表达水平与 PANSS 的一般病理症状分呈正相关(P<0.01)。同胞组与对照组合并统计,IL-1β与 TNF-α的 mRNA 表达水平呈正相关(r=0.847,P<0.01);IL-1β与 TH 的 mRNA 表达水平呈正相关(r=0.666,P<0.01)。患者组仅 IL-1β与 TNF-α的 mRNA 表达水平呈正相关(r=0.942,P<0.01)。结论精神分裂症患者 PBMC 细胞 TH、IL-1β和 TNF-α的 mRNA 表达水平高于正常,且与精神分裂症的一般病理症状显著相关。
Objective To explore the possible neuroimmunology mechanism in schizophrenia. Methods Thirty-nine patients with schizophrenia and their 25 siblings and 30 normal controls were recruited. The clinical symptom were evaluated by using the Positive and Negative Syndrome Scale （PANSS）, and the mRNA expression levels of interleukin-1β （IL-1β）, turnout necrosis factor-α （TNF-α） , and tyrosine hydroxylase （TH） in the peripheral blood mononuclear cell were measured with reverse transcription-polymerase chain reaction and semi-quantification technique. Results The mRNA expression levels of IL-1β, TNF-α and TH were higher in schizophrenic and their siblings than normal controls （ P 〈 0. 01 or 0. 05 ）. The correlation between the score of general symptom of PANSS and the mRNA expression levels of IL-1β （ r = 0. 420） , TNF-α （ r = 0. 430） was significant （ P 〈 0. 01 ）. The correlation between the mRNA expression levels of IL-1β and TH was found in controls （r = 0. 666, P 〈 0. 01）, but not in patients （P 〉 0. 05）, and the correlation between the mRNA expression levels of IL-1β and TNF-αwas significant in all groups （ r = 0. 847 or 0. 942, P 〈 0. 01 ）. Conclusion The mRNA expression levels of circulating TH, IL-1 β and TNF-α in schizophrenics are increased. Cytokine IL-1β and TNF-α may synergistically affect the multiple equivocal symptoms in schizophrenia. Imbalance between the synthesis of catecholamines and cytokines could possibly contribute to the pathogenesis of schizophrenia.
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2007年第4期209-212,共4页
Chinese Journal of Psychiatry
