摘要
目的通过在缺血/再灌注心肌中应用阿片受体激动剂吗啡和阻滞剂纳络酮,研究缺血后处理的保护机制和吗啡药物后处理的保护作用。方法SD大鼠60只随机分为6组行Langendorff心脏灌流:①停灌/再灌组(I/R);②纳络酮组(NAL);③缺血后处理组(Post-con);④纳络酮加后处理组(NAL+Post-con);⑤吗啡组(MOR);⑥吗啡加纳络酮组(MOR+NAL)。观测左室内压变化速率(±dp/dtmax),心肌梗死面积,冠脉流出液中肌酸激酶(CK)和乳酸脱氢酶含量(LDH),心肌超微结构改变。结果心脏复灌初期采取缺血后处理或灌注吗啡可以改善心功能,减小梗死面积。纳络酮对停灌/再灌心肌没有影响,但可削弱缺血后处理的心肌保护效果并完全取消吗啡的心肌保护作用。结论阿片受体的激活是缺血后处理心肌保护作用的机制之一。再灌之初给予吗啡能起到缺血后处理样的心肌保护作用。
Objective To investigate a possible role of opioid receptors in ischemic postconditioning and observe if morphine（MOR） has the protective effect on the isolated rat hearts following postconditioning.Methods Isolated hearts of 60 SD rats were perfused in Langendorff mode.And injury was induced by 30 min ischemia and 60 min reperfusion.After 30 min stabilization,hearts were divided into 6 groups randomly with 10 in each group：Ischemia/reperfusion（I/R） group：Isolated rat hearts underwent 30 min ischemia and 60 min reperfusion;Naloxone（NAL） group：Krebs-Henseleit solution containing 1 mol/L naloxone perfused during the 10 min before ischemia and the first 10 min of reperfusion;Postconditioning（Post-con） group：6 postconditioning cycles of 10 s reperfusion/10 s ischemia were applied at the beginning of 60 min reperfusion;Naloxone plus postconditioning（NAL＋Post-con） group：Both NAL and Post-con were applied;MOR group：Krebs-Henseleit solution containing 0.3 mol/L MOR perfused during the first 10 min of reperfusion;MOR plus naloxone（MOR＋NAL） group：Krebs-Henseleit solution containing 1 mol/L naloxone and 0.3 mol/L morphine perfused during the first 10 min of reperfusion.Hemodynamic data（±dp/dtmax）,myocardial infarct size,concentrations of lactate dehydrogenase（LDH） and creatine phosphokinase（CK） in the liquid of coronary and myocardial ultrastructure were assessed before and after ischemia.Results Postconditioning and MOR could remarkably improve heart function and reduce infarct size confirmed by concentrations of LDH and CK in the liquid of coronary,which could be revoked by Naloxone.Conclusion Cardioprotection of postconditioning may be mediated,in part,by active opioid receptors and MOR can offer cardioprotection as postconditioning when it is used at the beginning of reperfusion.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期610-613,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
缺血后处理
心肌再灌注损伤
阿片受体
心肌保护
吗啡药物后处理
ischemic postconditioning
ischemia/reperfusion injury
opioid receptor
myocardial protection
pharmacological postconditioning
