摘要
目的:探讨杏仁核点燃癫痫大鼠海马区损伤与胶质纤维酸性蛋白(GFAP)的表达及GFAP在实验性癫痫中的作用。方法:建立大鼠杏仁核点燃癫痫模型,应用光镜及电镜技术观察大鼠点燃癫痫后海马区的病理变化,同时应用免疫组织化学方法检测大鼠点燃癫痫后海马区GFAP免疫反应阳性细胞的细胞数、面积、周长和积分光密度。结果:实验组海马区光镜下可见神经细胞变性、肿胀、坏死及神经细胞脱失改变,电镜可见细胞核形态不规则、有切迹、核仁偏位、线粒体肿大、嵴断裂、膜破损和基质空化等改变,随癫痫发作次数增加上述改变越明显,对照组与手术对照组比较,各参数差异无显著性(P>0.05),将不同组别大鼠细胞数、面积、周长、积分光密度分别作单因素方差分析,不同组别间均差异有显著性(P<0.01),同时观察到海马区GFAP免疫反应阳性的胶质细胞随海马损伤的加重而GFAP免疫反应增强。结论:点燃癫痫鼠海马区损伤越重,胶质细胞GFAP免疫反应性越强,通过检测GFAP可以推测星形胶质细胞与癫痫后脑损伤的程度。
Objective To study the correlation between the injury in rat hippocampus after epilepsy kindled by amygdala and the expression of glial fibrillary acidic protein （GFAP）, and the role of GFAP in experimental epilepsy. Methods The rat epilepsy model was set up by electron amygdala, the pathological changes in hippocampus after kindled epilepsy were observed by using optical microscope and electron microscope. And the number of GFAP immunoreactive positive cells in hippocampus after rat kindled epilepsy, area, circumference and integral optical density were detected by immunohistochemistry. Results By using optical microscope, the neurocytes of hippocampus in experimental group were observed to denaturalize, tumefy, destroy and disappear. And under electron microscope, the karyon was irregular, nucleoles deviated, chondriosome tumefied, crista collapsed, membrane was damaged, substance cavitated. Such changes were more evident with the increased paroxysm of epilepsy, the parameters between control group and operation group had no difference （P〉 0.01）. But make a monofactorial variance analysis among cells＇ number, area, circumference and integral optical density in different groups, it showed that there were significant differences between optional two groups （P〈0.01）; at the same time, the more serious injury in hippocampus, the more powerful GFAP immunoreaction was.Conclusion The more serious injury in hippocampus, the more powerful GFAP immunoreaction is. The correlation between astrocyte and injury of brain after epilepsy can be speculated by detecting GFAP.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期837-840,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅资助课题(20030430)
关键词
点燃
癫痫
海马/损伤
胶质纤维酸性蛋白
kindling
epilepsy
hippocampus/injuries
glial fibrillary acidic protein
