摘要
从螺旋藻NaHCO3热水提取液中．得到四种多糖SP－1、SP－2、SP－3和SP－4，糖含量比为30：12：16：100．体外活性试验表明SP－4能促进T淋巴细胞的增殖．纸层析和高压液相色谱分析结果表明SP－4由L-鼠李糖、L-岩藻糖、L-阿拉伯糖、D-葡萄糖、D-葡萄糖胺和D-半乳糖醛酸组成，其摩尔比为4．94：2．99：2．96：1．00：1．26：2．33。IR光谱示848cm-1有明显的吸收峰，1HNMR分析在δ5．26附近有多个峰，过碘酸盐氧化和Smith降解表明SP－4分子中含有α（1→3）和α（1→4）糖苷键．
Four water-soluble polysaccharides were isolated from the hot NaHCO3 waterextract of the spirulina platensis. Their ratio of sugar contain was 30: 12: 16: 100 fromSP-1 to SP-4. Bioactivity experiment in vitro showed that SP-4 was able to enhance theproliferation of T lymphocyte. The polysaccharide SP-4 consisted of L-rhamnose, L-fucose, L-arabinose, D-glucose, D-glucoseamine and D-galacturonic acid by PC and HPLCanalyses, and their mole ratio was 4. 94: 2. 99 : 2. 96: 1. 00: 1. 26: 2. 33. It had a mainchain of α (l→3) and α (l→4) on the result of 848cm-1IR absortion peak, several peaksnear δ 5. 26ppm in 1H NMR spectrum, oxygenation of periodate and Smith degradation.
出处
《广州化学》
CAS
1997年第1期30-34,共5页
Guangzhou Chemistry
关键词
螺旋藻
免疫促进作用
多糖
spirulina platensis, immunologic enhancement, polysaccharide
