摘要
目的观察银耳多糖局部应用对创伤愈合的影响。方法①用Pruden法检测银耳多糖对大鼠腹部创口愈合后耐压力的影响;②直观观察银耳多糖对创口愈合周期的影响;③从创口分泌物中观察巨噬细胞出现时间和阳性率;④采用比浊技术测定创口愈合过程中受试动物血清C反应蛋白(CRP)含量的变化。结果①银耳多糖实验组大鼠腹部创口愈合后耐压力(84.56±10.50)mmHg明显高于对照组(72.31±11.23)mmHg(P<0.05);②银耳多糖缩短创面愈合时间,实验组为(7.55±0.38)d,对照组为(8.95±0.42)d,两组有非常显著性差异(P<0.001);③实验组创口分泌物出现巨噬细胞较对照组为早,其阳性率亦高于对照组;④创伤愈合过程中,实验组动物血清CRP于第1天与对照组均升高,第3、6天实验组明显下降,而对照组仍处于较高水平。结论银耳多糖局部应用具有保护创面,预防感染、缩短创伤愈合时间和增加创口愈合强度的作用。
Objective To observe the effects of local using tremella polysaccharides （TPS） on wound healing through animal experiment.Methods （1）Pruden＇s method was used to examine pressure-tolerances of healed wound inpacted by TPS;（2）the wound healing time of tested animals was inspected;（3）the appearance time and positive ratio of macrophage in smears of secretion from wounds were examined;（4） turbidity technic was used to examine the contents of C-reactive protein in serum of tested animals during the period of wound healing.Results （1）By Pruden＇s method, the pressure-tolerances of healed wounds were higher than the control, the differences were significant （P〈0.05）;（2）the wound healing times of tested animals were shorter than that of the control （7.55±0.38d vs 8.95±0.42d）, the differences were very significant（P〈0.001）;（3）the appearance time of macrophage in secretion smears of TPS-tested animals was earlier than the control, and the positive ratios of macrophage in tested animals with TPS were higher than the control, the differences were significant（P〈0.05）;（4）the contents of CRP in serum of TPS- tested animals obviously reduced 3, 6d after operation, but that of the control still remained at a high level, the differences were significant （P〈0.05）.Conclusion Tremella polysaccharides have the effects of protecting wound, preventing infection, shortening the wound healing time and strengthening the pressure tolerance of healed wound.
出处
《实用医药杂志》
2007年第8期965-967,共3页
Practical Journal of Medicine & Pharmacy
