摘要
目的:为低速离心方法用于微生物限度检查提供依据。方法:以3株细菌、2株真菌对低速离心方法进行回收率验证试验。结果:低速离心3～5min,枯草芽孢杆菌、大肠埃希菌、金黄色葡萄球菌的平均回收率在70%以上;黑曲霉、白色念珠菌平均回收率在70%以下。采用沉降法5min,黑曲霉、白色念珠菌的平均回收率在70%以上。结论:采用低速离心降低供试品溶液抑菌活性成分的方法,可用于细菌计数检查,不宜用于真菌计数检查。
Objective ：To provide a method for determining microbial limits by the method of low speed centrifuge. Methods ： Validation tests by the method of low speed centrifuge with 3 strains of bacterium and 2 strains of fungus. Results：The average recoveries were more than 70% when the method of low speed centrifuge（ time ：3 -5 min） was used with Bacillics subtilis,Escherichia coil and Slaphylococcus aureus ,but the average recoveries were less than 70% with Aspergillus niger and candida albicans, while applied with subside method for 5 min, the average recover- ies were more than 70% with those two microbes. Conclusion：Counting bacteria can use low speed centrifuge method which reduces antibiotic parts in tested materials, but counting fungi can not use this method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第10期1620-1622,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
微生物限度检查
细菌
真菌
低速离心
回收率
沉降法
microbial limit tests
bacteria
fungi
low speed centrifuge
recovery
subside method
