摘要
本文报告了利用放射性配基受体结合法分析测定了甲亢类阴虚和甲低类阳虚模型动物大脑中枢胆碱能M一受体的变化,以及养阴益气中药复方和温补肾阳中药复方对其影响,尝试从细胞生物学水平上对虚证的发病机理和中药复方治疗虚证的机制作些探讨。结果表明,甲亢类阴虚模型大鼠脑中枢胆碱能M一受体数量减少(P<0.05),素和力提高(P<0.05),养阴益气中药复方能提高其数量(P<0.05),降低其亲和力(P<0.05);相反,甲低类阳虚模型免大脑中枢胆碱能M一受体的数量增多(P<0.05),亲和力无明显改变,温补肾阳中药复方能降低其数量。提示中枢胆碱能M一受体的变化,可能是中医阴虚(甲亢)阳虚(甲低)病理生理功能改变的原因之一,中药对此具有纠正调整作用。
The binding of [~3H] quinuclidnyl benzilate(QNB) to membrane preparations of hyperthyroid rat brain(similar to yin-deficiency) and hypothyroid rabbit brain (similar to yang-deifciency) represents a specific interaction with muscarinic cholinergic receptor sites. In the hyperthyroid rat brain, the number of binding sites of the receptor decreased (P<0.05), while the affinity of the receptor was higher (P<0.05). In the hypothyroid rabbit brain, the number of binding sites of the receptor increased (P<0.05). It is found that tonics could regulate and correct the changes and the results suggested that the changes of muscurinic cholinergic receptor in the brain might be one of the characteristics occurring in case of yin-deficiency and yang-deficiency.
关键词
甲亢
甲减
补阴药
补阳药
HYPERTHYROIDISM/TCM therapy HYPOTHYROIDISM/TCM therapy YIN-REINFORCING AGENTS/Pharmacodynamics YANG-REINFORCING AGENTS/Pharmacodynamics RECEPTORS, CHOLINERGIC/Drug effects DISEASE MODELS, ANIMAL
