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FISH技术及其在环境微生物监测中的应用 被引量：11

Application of Fluorescence in Situ Hybridization in Monitor of Environmental Microbial Ecology
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摘要 荧光原位杂交(FISH)被广泛应用于微生物群落结构诊断和评价。利用FISH技术在环境样品上直接原位杂交,不仅可测定不可培养微生物的形态特征及丰度,而且可原位分析它们的空间及数量分布。在环境生物监测中有着广阔的应用前景。 Fluorescence in Situ Hybridization（FISH） was useful for many applications in diagnosis and assessment of the population structure of complex microbial communities and was a powerful tool for molecular ecology studies in microbiology. Using FISH, not only couled in this paper, the basic principle and processes of Fluorescent In Situ Hybfidi zation （FISH） were introduced, then the application status of FISH in study on biotreatment for sewage was strengthened. Finally, the future of this technology in China was expected.
作者 李冰冰 肖波 李蓓
机构地区 华中科技大学
出处 《生物技术》 CAS CSCD 2007年第5期94-97,共4页 Biotechnology
关键词 荧光原位杂交技术 环境微生物 监测 fluorescence in situ hybridization environmental microbe monitor
分类号 X172 [环境科学与工程—环境科学] Q939.9 [生物学—微生物学]
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参考文献28

  • 1Amann R.I.Molecular Microbial Ecology Manual:In situ identification of microorganisms by whole cell hybrization with rRNA targeted mucleic acid probes[J].Dordrecht:Kluwer Academic,1995,1-15.
  • 2Wagner M.,et al.In situ analysis of nitrifying bacteria in sewage treatment plants[J].Wat.Sci.Technol.,1996,34:237-244.
  • 3Wagner M.,Amann R.,et al.Identification and in situ detection of gram-negative filamentous bacteria in activated sludge[J].Syst.Appl.Microb.,1994,17:405 -417.
  • 4Wagner M.,G.Rath,R.Amann,etal.In situ identifi cation of ammonia -oxidizing bacteria[J].Syst.Appl.Microbiol.,1995,1:251-264.
  • 5Wagner M.,Noguera D.,et al.Combining fluorescent in situ hybridization with cultivation and mathematical modeling to study population structure and function of ammonia-oxidizing bacteria in activated sludge[J].Wat.Sci.Tech.,1998,37:441-449.
  • 6Manz W.,R.Amann,W.Ludwig,et al.Phylogenetic oigonucleotide probes for the major subclasses of proteobacteria:Problems and solutions[J].Syst.Appl.Microbiol.,1992,15:593-600.
  • 7Luxmy B.S.,et al.Analysis of bacterial community in membrane-separation bioreactore by fluorescent in situ hybridizaton and denaturing gradient gel electrophoresis[J].Wat.Sci.Technol.,2000,41:259-268.
  • 8DeLong E F,Wickham G S.Pace N R1 Phylogenetic stains:ribosomal RNA-based probes for the identification of single microbial cells[J].Science,1989,243:1360-1363.
  • 9梁毓,王树玉,贾婵维.荧光原位杂交技术的研究进展[J].中国优生与遗传杂志,2005,13(5):119-120. 被引量：12
  • 10Perry-O'Keefe H,S Rigby.K Oliveira.et al.Identification of indicator micro-organisms using a standardized PNA FISN method[J].J.Microbiol Methods,2006,47:281-292.

二级参考文献34

  • 1[22]Lee N P,Nielsen K H,Andreasen S,et al.Combination of Fluorescent In Situ Hybridization and Microautoradiography -- a New Tool for Structure-Function Analyses in Microbial Ecology[J].Appl.Environ.Microbio.,1999,65:1289-1297.
  • 2[1]DeLong E F,Wickham G S,Pace N R.Phylogenetic stains:ribosomal RNA-based probes for the identification of single microbial cells[J].Science,1989,243:1360～1363.
  • 3[2]Amann R,W Ludwig,K Schleifer.Phylogenetic Identification and In Situ Detection of Individual Microbial Cells without Cultivation[J].Microbio.Rev.,1995,59:143～169;
  • 4[3]Stahl D A.Application of phylogenetically-based probes to microbial ecology[J].Molecular Ecol.,1995,(4):535～542;
  • 5[4]Spear R N,Li S,Nordheim E V,Andrews J H.Quantitative imaging and statistical analysis of fluorescence in situ hybridization (FISH) of Aureobasidium pullulans[J].J.Microbiol.Methods,1999,35:101～110;
  • 6[5]Schuppler M,Wanger M,Scohn G,et al.In situ identification of nocardioform actinomycetes in activated sludge using fluorescent rRNA-targeted oligonucleotide probes[J].Microbiology,1998,144:249～259;
  • 7[6]De Los Reyes,M Hernandez,L Raskin.Quantification of Gordona amarae Strains in Foaming Activated Sludge and Anaerobic Digester Systems with Oligonucleotide Hybridization Probes[J].Appl.Environ.Microbio.1998,64:2503～2512;
  • 8[7]Patrick D,Jean P D,Rene M,et al.Contribution of molecular microbiology to the study in water pollution removal of microbial community dynamics[J].Re/Views in Environ.Sci.& Bio/Tech.,2002,(1):39～49;
  • 9[8]Wagner M R,Erhart W,Manz R,et al.Development of and rRNA-Targeted Oligonucleotide Probe Specific for the Genus Actinetobacter and its Application for In Situ Monitoring in Activated Sludge[J].Appl.Environ.Microbio.,1994,60:792～800;
  • 10[9]Rocheleau S C,Greer J,Lawrence C,et al.Differentiation of Methanosaeta concilii and Methanosarcina barkeri in Anaerobic Mesophilic Granular Sludge by Flourescent In Situ Hybridization and Confocal Scanning Laser Microscopy[J].Appl.Environ.Microbio.,1999,65:2222～2229;

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2007年 第5期

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