摘要
以Poly(I)-Poly(C)诱导鸡成纤维细胞Mx基因的表达,提取总RNA,RT-PCR扩增出全长Mx cDNA,扩增产物克隆入pMD19-T Simple载体中进行序列测定。结果表明:与GenBank公布的鸡Mx cDNA序列相比,其同源性达99.9%,为进一步研究鸡Mx基因的抗病毒活性和作用机理奠定了基础。
A full-length cDNA of chicken Mx gene was obtained using reverse transcription polymerase chain reaction （RT-PCR） amplification of total RNA extracted from chicken embryo fibroblast （CEF） which was induced with Poly（I）- Poly（C）. The RT-PCR product was subcloned into pMD19-T simple vector and the correctness of the sequences was confirmed by sequence analysis. Result of sequence analysis showed that its homology with Mx cDNA from GenBank was up to 99.9%. These data indicated that the full-length Mx cDNA was cloned correctly, which will play a great role in research of its antiviral function and mechanism.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第3期34-36,共3页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(30671509)
高等学校博士学科点专项科研基金资助项目(20061117004)
关键词
鸡
成纤维细胞
RT—PCR扩增
序列分析
chicken
chicken embryo fibroblast
RT-PCR amplification
sequencing
