摘要
[目的]测定A型口蹄疫病毒A/HeN/1/2009株全基因组序列并分析其基因特征,为研究我国最近发生的A型口蹄疫病毒的致病性、流行规律以及筛选适用的疫情防控疫苗株奠定分子基础。[方法]应用RT-PCR方法,分段扩增、克隆A/HeN/1/2009株基因,并进行基因测序;借助DNAStar分子生物学软件,从分子流行病学角度分析A/HeN/1/2009株与参考毒株之间可能的遗传衍化关系。[结果]该毒株基因组全长8 171 nts[不包括poly(C)区段和poly(A)尾巴],其中5'-UTR和3'-UTR分别为1 080、92 nts,蛋白编码区为6 999 nts。分析A/HeN/1/2009株遗传衍化关系显示,该毒株划为东南亚拓扑型Laos03系的VN09亚系,同源性依次为87.3%～91.8%、93.7%～94.5%和96.9%～98.4%。[结论]VP1中A24V、N85R、S196T,3A中I61V、T128S、E147G和A134V在该毒群的进化过程中扮演重要角色;A/SH/1/2009株VP1 140-160基序为RSD。
[Objective] The study was to sequence the whole genome of FMDV serotype A/HeN/1/2009 and analyze the characteristics contained in these gene sequences,this will be help us to study the pathogenicity and epidemicity of FMDV serotype A frequently occurred in these years and also be a great help to develop vaccines to prevent and control FMD.[Method] The related gene segments of A/HeN/1/2009 were amplified by RT-PCR and these genes were sent to sequencing company for sequencing.The epidemiological features of this serotype were analyzed by DNAStar.[Result] The whole genome was 8 171 nts[not including poly(C) section and poly(A) tail].The length of 5'-UTR,3'-UTR and protein coding regions were 1 080 nts,92 nts,and 6 999 nts respectively.The analysis of epidemiological features showed that A/HeN/1/2009 was the VN09 subline belong to southeast Asia topology type Laos03 system,the homologies in turn were 87.3%-91.8%,93.7%-94.5%,96.9%-98.4%.[Conclusion] A24V,N85R,S196T of VP1 protein,I61V,T128S,E147G and A134V of 3A protein played key role in the pathogenicity of FMDV serotype A.We also found that the motif sequence of VP1 in A/HeN/1/2009 was RSD.
出处
《安徽农业科学》
CAS
2013年第2期640-641,700,共3页
Journal of Anhui Agricultural Sciences
基金
科技部国际合作重点项目(2005DFA30720)
