摘要
目的探讨氯化镉诱发人支气管上皮细胞系(16HBE)恶性转化过程中hMSH2基因mRNA动态变化的规律。方法用反转录-聚合酶链反应(PT-PCR)技术检测16HBE、氯化镉恶性转化16HBE系不同阶段(第5、15、32代细胞及成瘤细胞)hMSH2基因mRNA的表达情况。结果随着转化代数的增加,hMSH2基因mRNA的表达逐渐降低,到第32代细胞后表达明显下降(P<0.01)。结论氯化镉诱发16HBE系恶性转化过程中hMSH2基因表达逐渐下降,hMSH2基因水平的改变可能是氯化镉的致癌机制之一。
Objective To explore the expression of hMSH2 mRNA during the malignant transforming stages of 16HBE cells induced by cadmium chloride. Methods Reverse transcription-polymerase chain reaction （RT-PCR） was used to measure the expression ofhMSH2 mRNA in 16HBE cells and its different passage cells treated by cadmium chloride （the 5th , 15th, 32th passage, and neoplasmed cells from nude mice＇ s tumor tissue）. Results With the passages of 16HBE cells treated with cadmium chloride increased, the expression of hMSH2 gene decreased gradually （ P 〈 0.01 ）. Conclusion During transformation from 16HBE cells to malignant transformation induced by cadmium chloride, the expression of hMSH2 gene decreased, the alteration of hMSH2 gene may be a possible carcinogenic mechanism for cadmium chloride.
出处
《中国职业医学》
CAS
北大核心
2007年第5期375-377,共3页
China Occupational Medicine
基金
国家自然科学基金资助项目(30771781)
广东省自然科学基金资助项目(06022672)
广州市科技攻关重点资助项目(2003Z2-E0191/E0192)
广州市属高校科技计划项目(1002)
