摘要
探讨肿瘤坏死因子α（ＴＮＦα）在自身免疫性甲状腺炎发病过程中的作用，为深入对该病诊断、治疗的认识提供理论依据。方法分别用酶联吸附法（ＥＬＩＳＡ）、生物活性法检测自身免疫性甲状腺炎患者２５例、Ｇｒａｖｅｓ病患者９例、正常人１８例的外周血单个核细胞（ＰＢＭＣ）体外诱生ＴＮＦα的能力。结果自身免疫性甲状腺炎患者ＴＮＦα活性显著高于正常对照组（Ｐ＜０．００１），Ｇｒａｖｅｓ病患者ＴＮＦα活性低于正常对照组（Ｐ＜０．０１）。两种方法所测ＴＮＦα有较好相关性（ｒ＝０．９７２、Ｐ＜０．００１）。自身免疫性甲状腺炎患者ＴＮＦα与血清总三碘甲状腺原氨酸（ＴＴ３）、游离三碘甲状腺原氨酸反相关（ｒ分别为－０．４３７、－０．４２３，Ｐ均＜０．０５）与抗甲状腺过氧化物酶抗体（ＴＰＯ－Ａｂ）、抗甲状腺球蛋白抗体（ＴＧＡ）正相关（ｒ分别为０．５３、０．５１，Ｐ均＜０．０１）；与患者病程、甲状腺肿大无关。
Objective To observe the action of tumor necrosis factor α(TNFα)in the pathogenesis of autoim mune thyroiditis.Methods The activity of TNFα of peripheral blood mononuclear cells was detected in 25 cases of autoimmune thyroiditis,9 cases of Grave`s disease,and 18 normal controls by ELISA and bioassay.Results The activity of TNFα in patients with autoimmune thyrioditis was signficantly higher than that in normal controls( P <0.001),and in patients with Grave′s diseases significantly lower than that in normal controls( P <0.01)。TNFα activity detected by two methods showed a good correlation (r= 0.972 P <0.001).There were anegative correlation between TNFα and TT3 or FT3(r= -0. 437 ,-0.432 respectively,both P <0.05)and apositive correlation between TNFα and TPO-Ab or TGA(r= 0.53,0.51 respectively,both P <0.01).TNFα activity was not associated with the period of disease and the size of thyriod.Conclusion TNFα exerts an important role in the pahtogenesis of autoimmune thyroiditis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第5期378-381,共4页
National Medical Journal of China
