摘要
目的研究HO-1对氧糖剥夺海马神经元线粒体运动调节蛋白的影响.方法将培养7 d的大鼠海马神经元随机分为4组:正常培养组(C组)、正常培养+血晶素组(C+H组)、氧糖剥夺组(D组)、氧糖剥夺+血晶素组(D+H组).C组正常培养方法培养.C+H组在正常培养的神经元培养液中加入血晶素使其终浓度为10μmol/L后按正常培养24 h.D组神经元进行缺糖、缺氧后复糖、复氧处理.D+H组神经元用10μmol/L血晶素处理24 h后进行缺糖、缺氧后复糖、复氧处理.进行神经元纯度鉴定,细胞存活率,HO-1-mRNA表达,HO-1、Miro1、Miro2和Milton蛋白表达,神经元凋亡的检测.结果 C+H组神经元HO-1-mRNA和HO-1表达高于C组(P<0.01),Miro1、Miro2和Milton蛋白表达高于C组(P<0.01),神经元存活率和神经元凋亡率变化无统计学差异(P>0.05);D组神经元HO-1-mRNA和HO-1表达高于C组(P<0.01),Miro1、Miro2和Milton蛋白高于C组(P<0.01),神经元存活率低于C组(P<0.01),神经元凋亡率高于C组(P<0.01);D+H组神经元HO-1-mRNA和HO-1表达高于D组(P<0.01),Miro1、Miro2和Milton蛋白高于D组(P<0.01),神经元存活率高于D组(P<0.01),神经元凋亡率低于D组(P<0.01).结论 HO-1增加海马神经元线粒体运动调节蛋白Miro1、Miro2和Milton的表达,抑制神经元的凋亡.
Objective To investigate the effects of hemeoxygenase-1(HO-1)on mitochondrial movement protein expression in primary cultured rat hippocampus neurons after oxygen glucose deprivation.Methods Primary rat hippocumpus neurons cultrured for 7 days were dividend into four groups.Experimental group cells were respectively carried out 10μmol/L Heme precondition(group C+H),OGD(group D),10 μmol/L Heme precondition + OGD(group D+H),Control cells were cultured normally(group C).Compound remained present throughout the duration of the experiment until analysis 24 h later.Neuron viability and apoptosis were weasured.The expression of HO-1,Miro1,Miro2,Milton protein and HO-1-mRNA were detected.Results The protein and mRNA expression levels of HO-1 in group C+H were higher than group C(P<0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group C(P<0.01).The neuron viability and apoptosis had no significant difference between group C+H and group C(P>0.05).The protein and mRNA expression levels of HO-1 in group D were higher than group C(P<0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group C(P<0.01),the neuron viability rate was lower and neuron apoptosis rate was higher than group C(P<0.01).The protein and mRNA expression levels of HO-1 in group D+H were higher than group D(P<0.01),the protein expression levels of Miro1,Miro2 and Milton were higher than group D(P<0.01),the neuron viability rate was higher and neuron apoptosis rate was lower than group D(P<0.01).Conclusion HO-1 can inhibit the apoptosis of hippocampus neurons induced by oxygen glucose deprivation through increasing mitochondrial movement proteins expression.
出处
《昆明医科大学学报》
CAS
2012年第4期4-7,共4页
Journal of Kunming Medical University
基金
云南省科技厅面上基金资助项目(2008CD120)
昆明医学院第一附属医院博士科研启动基金资助项目(2007bs10)
