摘要
目的:建立测定酸牛奶中纳他霉素含量的反相高效液相色谱法。样品用甲醇萃取并沉淀蛋白质后进样,用ODS-2(250mm×4.6mm,5μm)色谱柱分离,流速为1.0mL/min,流动相:0.053mol/L乙酸铵与0.025mol/L氯化铵的缓冲液∶乙腈为70∶30(体积比)1L+5mL四氢呋喃,紫外检测波长302nm,RSD为0115%(n=10),标准曲线r=0.9962,平均回收率分别为96.5%。结论:本法具有操作简单、快速测定、灵敏度高、专属性好和准确性好等优点。
RP-HPLC method of determination of natamycin in yoghurt was developed. The sample was separated at room temperature on a ODS-2 column （250mm× 4.6mm,5μm） eluted at a flow rate of 1.0 mL/min with a mobile phase of 1L（0.053mol/L NH4Ac ＋0.025mol/L NH4CL ） - Acetonitrile （ 70 ： 30, V/V ） ＋ 5mLTetrahydrofuran. Natamycin was detected and quantified by the absorbance at 302nm, This method is simple, accurate, highly specific and reliable for the determination of natamycin,
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第10期216-218,共3页
Science and Technology of Food Industry
