摘要
为探讨HIV3B和HIV Ada-M是否能感染培养的人背根神经节(DRG)神经元,我们建立了人DRG器官型培养和分散培养模型。DRG组织块培养14 d后,用游离的HIV3B或HIV Ada-M病毒颗粒处理并继续培养14 d,用倒置相差显微镜定期观察神经元突起的生长和形态变化。分散的DRG神经元培养3 d后,用游离的HIV3B或HIV Ada-M病毒颗粒处理并继续培养3 d。终止培养后,行微管相关蛋白2(MAP2)免疫荧光染色,然后利用荧光显微镜观察神经元突起的变化情况。DRG组织块培养28d、分散的DRG神经元培养6 d后,用电子显微镜观察神经元超微结构的改变。未用HIV处理的标本作为对照。在器官型培养的DRG神经元突起内发现了未成熟的HIV样病毒颗粒。在分散培养的DRG神经元突起内发现了大量的HIV样病毒颗粒。但HIV感染并不影响两种培养神经元的形态和超微结构的改变。以上结果对于进一步研究HIV感染以及与HIV感染有关的周围神经病变的病理发生机制具有重要意义。
To investigate whether HIV3B and HIV Ada-M can infect cultured human dorsal root ganglion （DRG） neurons, organotypic and dissociated human fetal DRG cell culture models were established. On the 14th day, organotypic cultured DRG explants were exposed to HIV3B or HIV Ada-M for another 14 days. Outgrowth and morphology of neurites were observed with phase contrast microscope at different time of cultured age. On the 3rd day, dissociated cultured DRG cells were exposed to HIV3B or HIV Ada-M for another 3 days. After that, dissociated DRG cells were processed for microtubule-asseciated protein 2 （MAP2） labeling and observed with fluorescent microscopy. DRG explants on the 28th day and dissociated DRG cells on the 6th day, the samples were processed for electronic microscopic observation. Both organotypic and dissociated DRG cultures were cultured continuously in culture media as controls. Immature HlV-like particles were found in organotypic cultured DRG neurons. Many HIV-like particles were found in dissociated cultured DRG neurons. HIV infection could not cause morphological and ultrastructural alterations on both organotypic and dissociated cultured DRG neurons. These dis- coveries will be valuable for studies on pathogenic mechanisms of HIV infection and/or HIV associated peripheral neuropathies.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2007年第5期536-541,共6页
Chinese Journal of Neuroanatomy
基金
教育部留学回国人员科研启动基金(外教司留[2003]406号)
山东省优秀中青年科学家奖励基金(02BS091)资助项目
