摘要
对１６～１８ｄ胎龄的大鼠皮层细胞进行分离培养，观察过量（１０μｍｏｌ／Ｌ）谷氨酸对皮层神经元的影响以及氯胺酮的保护作用。结果表明：培养１０ｄ的神经元置于１０μｍｏｌ／Ｌ谷氨酸和低糖（ｌｇ．／Ｌ）ＤＭＥＭ培养液中后，随着作用时间延长，乳酸脱氢酶（ＬＤＨ）活性增加。如在谷氨酸处理前，于培养液中加入ＮＭＤＡ受体非竞争性拮抗剂氯胺酮，则ＬＤＨ活性明显低于对照组。结果表明：１０μｍｏｌ／Ｌ谷氨酸能造成培养的神经元严重损伤，氯胺酮对此有明显的保护作用。
The toxic effect of glutamate on cultured cortical neurons and the protective effect of ketamine were studied on the culture of cortical neurons from 16 to 18-day-old fetal rats,The culture of 10 days was exposed to 10μmol/L glutamate and low glucose(1g/L).Dynamic observation at 10 min,3h and 24h showed that the release of lactate dehydrogenase(LDH)into the culture supernatant was a function of time,when the cell culture was pretreated with ketamine( 0～500 μmol/L)10 min beforehand,the neurotoxic effect of glutamate(10μmol/L at 3h)wasobviously decreased,These results demonstrate that the cultured corticalneurons from fetal rat are seriously damaged by 10μmol/L glutamate and such a damage is attenuated by ketamine,suggesting that ketamine may protect neurons from glutamate injury.
出处
《徐州医学院学报》
CAS
1995年第3期221-224,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金
关键词
谷氨酸
氯胺酮
神经元
毒性
脑缺血
ketamine
glutamate
cortical neuron
cell culture
