摘要
目的:采用GC和GC-MS色谱法研究并建立各产地乌骨藤药材脂溶性成分的指纹图谱。方法:采用毛细管气相色谱分析,DB-5石英毛细管柱(0.25 mm×30 m,0.25μm);程序升温,柱温70℃(保持5 min),以8℃.min-1升至200℃(保持12 min),再以5℃.min-1升至260℃(保持10 min);以高纯氮为载气;氢火焰离子化检测器(FID),检测器温度300℃;气化室温度280℃;分流比10∶1。结果:乌骨藤药材指纹图谱选定了15个共有峰,质谱分析鉴定出其成分,并对不同产地药材进行聚类分析及相似度评价。结论:本法重现性好,可结合乌骨藤药材的HPLC指纹图谱评价其质量。
Objective：To study characteristic fingerprints of the fat-soluble components of different Marsdenia tenacissima samples by GC and GC-MS.Method： The sample was split in the 280 ℃ injection port,with 10∶1 split ratio,and separated on a fused silica capillary column（DB-5,30 m×0.25 mm I.D.,0.25 μm film）.The temperature program was 70 ℃ for 5 min,8 ℃·min^-1 to 200 ℃,then 5 ℃·min^-1 to 260 ℃,260 ℃ for 10 min,The high pure nitrogen was used as a carrier gas,The FID temperature was 300 ℃.Result：There are 15 common peaks in the GC fingerprints of M.tenacissima and they are identified by GC-MS.The analyses on cluster and similarity degree of the finger the crude drugs from various habitats have been performed.Conclusion： The method has good repeatability.The GC fingerprint combined with the HPLC fingerprint of M.tenacissim can be used to evaluate its quality integrally.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第20期2146-2149,共4页
China Journal of Chinese Materia Medica
基金
江苏省自然科学基金项目(BK2005100)
