摘要
目的通过吸入甲苯对小鼠神经的行为学影响,探讨其神经毒性及成瘾性的效果和机制。方法吸入染毒,用1.21,2.42,4.84,9.68,19.36 mg/L 5个不同浓度下的甲苯染毒5 d,20 min/d,观测染毒后小鼠自主活动次数、水迷宫潜伏期和盲端数、条件性位置偏爱(conditioned place preference,CPP)的变化。结果吸入2.42,4.84 mg/L浓度甲苯的小鼠,自主活动次数与对照组比较,明显增加(P<0.05);而吸入19.36 mg/L浓度甲苯的小鼠,自主活动次数与对照组比较,明显减少(P<0.05)。仅有吸入19.36 mg/L浓度组的小鼠,水迷宫潜伏期与对照组比较,明显延长(P<0.05),盲端数无明显变化。吸入2.42,4.84,9.68 mg/L浓度甲苯的小鼠,对甲苯产生了CPP(P<0.05)。结论吸入甲苯的小鼠,在低浓度下,能明显增强自主活动性;在高浓度下,能明显减弱自主活动性。仅在高浓度下明显降低小鼠的学习记忆功能;并在一定浓度下,使小鼠对甲苯产生精神依赖。
Objective Through investigating the neurobehavioral effects induced by inhaling toluene to probe into its neurotoxicity and effects and mechanism of addiction. Methods Through inhaling, expose mice to toluene of 1.21, 2.42, 4.84, 9.68, 19.36 mg/L 20-rain daily for 5 days. The changes of their autonomic activities, the ability of study and memory and conditioned place preference （CPP） were observed after exposures. Results The number of autonomic activities increased obviously in 2. 4？ and 4.84 mg/L groups （P〈0.05）, while decreased significantly in 19.36 mg/L group when compared with the control group （P〈0.05）. Only in 19.36 mg/L group the latency period of water maze prolonged obviously（P〈0.05）, however, the number of entering into dead end of water maze had no significant change. The 2.42, 4.84 and 9.68 mg/L groups demonstrated CPP to toluene（P〈0.05）. Conclusions Toluene could increase autonomic activities at lower doses and decrease activities at higher exposure levels in mice. Only under higher doses it could cause the function of study and memory of mice to descend obviously and produce a clear psychological dependence at certain level in mice.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2007年第5期270-273,共4页
Industrial Health and Occupational Diseases
关键词
甲苯
自主活动
学习记忆
CPP
小鼠
Toluene
Autonomic activities
Study and memory
Conditioned place preference
Mouse
