摘要
对适合南方地区冬季种植的纤用亚麻品种组织培养过程中基本培养基、激素配比、外植体材料的基因型和苗龄以及再生不定芽的生根条件进行了比较研究。结果表明,适合于亚麻白花品种组织培养的最佳培养基为YB1,不定芽诱导率可达98.50%。在此培养基上,白花、黑亚4号、K6531、K7697、HI026、HI045、I039和阿丽亚那下胚轴不定芽的诱导率分别为98.50%、98.50%、56.50%、42.47%、54.40%、0、27.13%和97.30%,平均出芽数为11.43、9.33、2.17、0.77、1.10、0、0.90和10.68。苗龄为7-10天的下胚轴最适于诱导不定芽,随苗龄增加,不定芽的诱导率呈下降趋势。RB5培养基最适于不定芽的生根,生根率达100%,平均生根数为15.3。实验还确定了亚麻对卡那霉素、氨苄青霉素和头孢霉素的抗性浓度阈值。
Flax is an important economic crop for fiber and/or oil in China. An efficient flax plant regeneration system was established through optimization of basal medium, hormone combination, flax genotype and age of hypocotyl explants. The best medium for callus and adventitious shoot differentiation was YB1. Shoot induction rate of Baihua, Heiya NO.4, K6531, K7697, HI026, HI045, 1039 and Aliyana was 98.50%, 98.50%, 56.50%, 42.47%, 54.40%, 0, 27.13%, and 97.30%, respectively, and mean shoot number per explant was 11.43, 9.33, 2.17, 0.77, 1.10, 0, 0.90, and 10.68, respectively. Hypocotyls taken from seedlings 7 to 10 days old showed the highest regeneration ability. RB5 medium was optimal for root formation of regenerated shoots, with 100% root induction and 15.3 roots per shoot. Kanamycin at 60 mg.L 1 inhibited callus and shoot induction completely; the effect of ampicillin and cefotaxime at the concentrations tested was not significant.
出处
《植物学通报》
CSCD
北大核心
2007年第5期629-635,共7页
Chinese Bulletin of Botany
基金
中国农业科学院杰出人才科研启动经费
关键词
不定芽
下胚轴
亚麻
再生体系
adventitious shoot, hypocotyls, Linum usitatissimum L., plant regeneration
