摘要
目的探讨FAS/FASL基因多态性在煤工尘肺患者发病遗传易感性中的作用及其与煤工尘肺纤维化程度的联系。方法选择340例汉族煤工尘肺为观察对象,312例汉族煤尘接触者为对照组,应用多聚酶链反应-限制片段多态性(PCR-RFLP)技术检测FAS-1377G>A、FAS-670A>G位点及FASL-844T>C位点的基因多态性。结果煤工尘肺组FAS-1377、FAS-670及FASL-844各基因及等位基因分布频率与对照组比较,差异无统计学意义(P>0.05)。以接尘工龄≥25年为对照,工龄<25年的煤工尘肺患者FAS-1377GA/AA型者发生尘肺的危险性是GG型的1.463倍(P=0.098,95%CI:0.932～2.298);FAS-670AG型发生尘肺的危险性是GG型者的1.494倍(P=0.098,95%CI:0.928～2.404);FASL- 844 TT型和TC型发生尘肺的危险性分别是CC型的5.455倍(P=0.039,95%CI:1.088～27.358)及1.338倍(P=0.098,95%CI:0.852～2.101)。接尘工龄<25年FASL-844基因分布频率和工龄≥25年比较,差异有统计学意义(P<0.05),FASL-844TT型发生煤工尘肺的危险性是CC/TC型的4.810倍(P=0.054,95%CI:0.971～23.833);接尘工龄≥25年为对照,工龄<25年组FASL-844 TF/CT+FAS-1377GA型发生煤工尘肺的危险性是FASL-844CC+FAS.1377GG型的1.810倍;FASL-844TF/CT+FAS-670AG型是FASD-844CC+ FAS-670AA型的2.117倍;FASL-844TT/CT+FAS-1377GA/AA+FAS-670AG/GG型发生煤工尘肺的危险性是FASL-844CC+FAS-1377GG+FAS-670AA型的2.043倍。结论FAS-1377G>A、FAS-670A>G及FASL- 844T>C 3个位点的基因多态性在中国汉族煤工尘肺发病的遗传易感性中不起主要作用,但这3个多态性位点及位点的联合作用对病变的发展有影响。
Objective To investigate the effects of FAS and FASL gene polymorphisms on genetic susceptibility of coal worker＇ s pneumoconiosis and their relationship to the pulmonary fibrosis. Methods 340 with coal worker＇s pneumoconiosis （CWP） and 312 coal mine workers （controls） exposed to the coal dusts were selected. FAS-1377G〉A, FAS-670A〉G and FASL-844T〉C gene polymorphisms were analyzed by PCR-RFLP techniques. Results The distribution frequencies of genotypes of FAS-1377, FAS-670, FASL-844 genotypes in CWP had no significant differences compared to the control. Compared to CWP patients with exposure year≥25, the risk of pneumoconiosis with FAS-1377 GA/AA genotype was significantly higher than those with FAS-1377GG in the patients working age 〈25 years （P=0.098; 95%CI：0.932-2.298）;the risk of CWP in those with FAS-670AG genotype was higher than those with FAS-670GG genotype （P=0.098; 95%CI： 0.928-2.404）; the risks of CWP in those with FASL-844TT genotype and FASL-844TC genotype were respectively higher than those with FASL-844CC genotype （P=0.039, 95%CI： 1.088-27.358; P=0.089, 95%CI： 0.852-2.101）. The frequencies of genotypes of FASL-844T〉C were significantly different between CWP patients with exposure year ≥25 and 〈25. The risk of CWP with FASL-844TT genotype was significantly higher than that of FASL-844rIT＋TC （P=0.054, 95%CI：0.971-23.833）. The risk of CWP patients with FASL-844TT/CT＋FAS-1377GA genotype was 1.810-fold than the patients with FASL-844CC＋FAS-1377GG genotype. The risk of CWP patients with FASL-844TT/CT＋FAS-670AG genotype was 2.117-fold than the patients with FASL-844CC＋FAS-670AA genotype. The risk of CWP patients with FASL-844TT/TC＋FAS-1377GA/AA＋FAS-670AG/GG genotype was 2.043-fold than the patients with FASL-844CC ＋FAS-1377GG ＋FAS-670AA genotype. Conclusion FAS- 1377G〉A, FAS-670A〉G and FASL-844T〉C gene polymorphisms may not be associated with the susceptibility of CWP in Han nationality, but these three gene polymorphisms and their joint actions may influence on the progression of CWP.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第9期532-536,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
江苏省卫生厅科研课题(H200628)
南京医科大学校重点项目(2005NYDZD08)
关键词
尘肺
基因
多态性
单核苷酸
Pneumoconiosis
Gene
Polymorphism, singl mucleoticle
