摘要
[目的]探讨国内外的24份普通小麦品种间的遗传差异。[方法]以改进的酚-氯仿法提取国内外24份普通小麦品种基因组DNA,采用Genomic-SSR和EST-SSR标记技术分析24份小麦品种的遗传多样性,并对2种评价方法进行分析比较。[结果]采用37对Genomic-SSR引物对24份小麦基因型的38个位点进行扩增,24个位点共扩增出152个多态性片段,每个位点上平均片段数为5.85。采用67对EST-SSR引物对24个小麦基因型的78个位点进行扩增,37个位点共扩增出120个多态性片段,每个位点平均片段数为3.24.24份小麦品种的综合遗传距离为0.1541～0.7820,平均为0.4231。[结论]EST-SSR标记能更准确地反映出不同小麦基因型之间遗传差异和亲缘关系。
[Objective] The research aimed to discuss the genetic diversity among 24 common wheat varieties at home and abroad. [Method] Improved phenol-chloroform method was used to extract genomic DNA from 24 common wheat varieties at home and abroad. Genomic-SSR and EST-SSR marker technologies were adopted to analyze the genetic diversity of 24 wheat varieties and two evaluation methods were analyzed and compared. [Result] When 37 pairs of Genomic-SSR premiers were used to amplify 38 sites in 24 wheat genotypes, 152 polymorphic segments were obtained from 24 sites, with average segments number per site of 5.85. When 67 pairs of EST-SSR premiers were used to amplify 78 sites in 24 wheat genotypes, 120 polymorphic segments were obtained from 37 sites, with average segments number per site of 3.24. Comprehensive genetic distance was 0.1541～0.7820, being 0.423 1 on average. [Conclusion] EST-SSR marker could reflect the genetic difference and relationships among different wheat genotypes exactly.
出处
《安徽农业科学》
CAS
北大核心
2007年第26期8173-8175,8183,共4页
Journal of Anhui Agricultural Sciences
基金
国家科技攻关项目(2004BA505B03)。
