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小麦EST-SSR标记的开发、染色体定位和遗传作图 被引量:36

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摘要 利用普通小麦EST数据库(GenBank/dbEST)中最新的151695条EST序列(2003.7.14~2004.8.24)进行了微卫星序列的筛选, 共发现微卫星序列2038个, 占整个EST数据库的1.34%. 根据筛选得到的微卫星序列设计并合成了249个引物对. PCR扩增结果表明, 166个引物对在不同小麦品种中显示出稳定清晰的带型, 可以作为小麦和相关物种的新的EST-SSR分子标记. 将其中的93个引物对、193个位点定位到除4A和4B外的19条特定的染色体. 利用RIL群体将43个EST-SSR位点绘制到遗传图谱的11条染色体上.
出处 《科学通报》 EI CAS CSCD 北大核心 2005年第20期2208-2216,共9页 Chinese Science Bulletin
基金 国家高技术研究发展计划〔批准号:2003AA207100) 国家自然科学基金(批准号:30571155)资助项目.
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二级参考文献3

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