摘要
介绍了蜜糖文心兰的组织培养体系与工厂化生产技术,指出用花梗和侧芽作外植体进行组织培养,可从再生芽中诱导出类原球茎,再通过芽和类原球茎2种途径进行增殖。在培养中观察发现,发育粗壮但未分化假鳞茎的再生芽诱导类原球茎能力最强,发育程度不同的类原球茎增殖特性不一,愈伤态类原球茎具较强增殖能力,增殖率最高;通过液体振荡培养7 d可以促进增殖潜能的形成,淘汰发育不良褐化死亡的类原球茎,使类原球茎发育同步化,通过悬浮培养后类原球茎出苗整齐一致,提出了液体悬浮培养和固体培养结合的高效工厂化生产新程序。
Tissue culture and industry production technology of Oncidium ＇Sweet Sugar＇ were introduced in this paper. It pointed out that using scape nodes and axillary bud as explants could successfully induced protocorm-like bodies （Plbs） from the regenerated shoots. Two ways of proliferations through shoots and Plbs could be found in the culture. Those shoots that the pseudobu]b had not obviously been differentiated had higher potential in induction of the Plbs. Different types of the Plbs showed different proliferation characteristics, one of which like embryogenic callus with yellow-green color had strong ability of proliferation. Suspension culture in the liquid medium in Shaker for 7 days could increase the potential of proliferation, make Plbs to develop synchronously and eliminate some abnormal Plbs. An innovative technique of micropropagation integrated with suspension and solid cultures for industry production was developed based on the experiments.
出处
《广东农业科学》
CAS
CSCD
2007年第10期35-38,共4页
Guangdong Agricultural Sciences
基金
广东省自然科学基金项目(5006236)
广东省科技攻关项目(2005B20901001)
关键词
文心兰
快速繁殖
悬浮培养
类原球茎
Oncidium
micropropagation
suspension culture
protocorm-like bodies
