摘要
目的:探讨Cyclin D1和p27kip1蛋白在胃黏膜癌变过程中的表达及相互关系。方法:采用免疫组化SP法分别检测慢性浅表性胃炎(chron-ic superficial gastritis,CSG)、慢性萎缩性胃炎(chronic atrophic gastritis,CAG)、肠化生(intesti-nalm etaplasia,IM)、不典型增生(dysplasia,DYS)和胃癌(gastric carcinoma,GCA)组织中Cyclin D1和p27kip1的表达变化。结果:Cyclin D1的过表达随病变发展表达率逐渐增高,CAG组与CSG组比较,差异无统计学意义,P>0.05;IM、DYS、GCA组与CSG组比较,差异均有统计学意义,P均<0.05。p27kip1在上述黏膜中的表达呈递减趋势,CSG中最高,GCA组最低,CAG组和CSG组比较,差异无统计学意义,P>0.05;IM、DYS、GCA组与CSG组比较,差异均有统计学意义,P<0.05。Cyclin D1和p27kip1的表达呈负相关,γ=-0.53,P=0.000。结论:胃黏膜癌变过程中的Cyclin D1阳性表达呈递增趋势,p27kip1表达呈递减趋势;Cyclin D1和p27kip1表达变化发生在胃黏膜癌变早期,两者在胃癌黏膜癌变过程中的表达呈负相关提示两者可能存在相互抑制机制。
OBJECTIVE： To evaluate the relationship between the expression of Cyclin D1 and p27^kip1 in the canceration course of the stomach. METHODS： The immunohistochemical staining technique （SP method） was used to detect the expressions of Cyclin D1 and p27^kip1 in chronic superficial gastritis （CSG）, chronic atrophic gastritis （CAG）, intestinal metaplasia （IM）, dysplasia （DYS）, gastric carcinoma （GCA） biopsy specimens. RESULTS： The positive Cyclin D1 expression rates increased with the progressing from CAG→IM→DYS→GCA, respectively, and those in IM, DYS and GCA were different from those in CSG, P〈0. 05, while DYS group was indifferent from GCA group P〉0.05. The positive p27^kip1 expression rates decreased with the mucosa progressing from CAG→IM→ DYS→ GCA. There was a negative correlation between the expression of Cyclin D1 and p27^kip1 （r= -0. 53 ,P=0. 000）. CONCLUSIONS： The expression rates of Cyclin D1 in the canceration course of the stomach mucosa are increased and those of p27^kip1 are decreased. The abnormal expressions of them are found in the early term of the canceration course of the stomach mucosa, and the inverse expression between Cyclin D1 and p27^kip1 suggests antagonism between the two.
出处
《中华肿瘤防治杂志》
CAS
2007年第22期1700-1702,共3页
Chinese Journal of Cancer Prevention and Treatment
