摘要
为探讨Ⅲ型前胶原（ｈｐｃⅢ）与层粘连蛋白（ＬＮ）在肝纤维化诊断中的意义，应用放免法检测Ⅲ型前胶原和ＥＬＩＳＡ法检测层粘连蛋白对２０８例不同类型的肝炎病人和３０例正常人的血清进行分析。结果提示：慢性肝炎、肝硬化病人血清中ｈｐｃⅢ和ＬＮ明显升高，与正常人比较有显著差异（Ｐ＜０．０１或Ｐ＜０．０５），ｈｐｃⅢ在慢性肝炎阶段已有明显增高，且随肝炎的进展程度而递增，与门静脉宽度呈正相关，ｒ＝０．８２，与食道静脉曲张不相关，说明ｈｐｃⅢ在肝纤维化早期既已有明显改变；ＬＮ与肝功检查不相关，与门静脉宽度和食道静脉曲张程度呈正相关，相关系数分别为０．７９，０．９３，说明ＬＮ是反映肝纤维化晚期的良好指标，二者联合检测，不仅能提高肝纤维化的灵敏度、特异性，且能早期诊断，尤其能反映肝纤维化演变过程。
We detected the serum concentrations of hpcⅢ (type Ⅲ procollagen) and LN (laminin) in 208 patients with all kinds of hepatitis and in 30 normal donors by radioimmunoassay and ELISA. The results were as follows: There was a significance difference between serum concentrations of hpcⅢ and LN in normals as compared to patients with chronic hepatitis and hepatic cirrohosis (P<0.01 or P<0.05). In chronic hepatitis stage, hpcⅢ increased and more high gradually by the progress of disease. The serum concentrations of hpcⅢ showed a good correlation (r=0.82, P<0.01) with portal hypertension and no correlation with varix of esophagus. The serum concentration of LN showed a good correlation (r=0.79, 0.93;P<0.01) with not only portal hypertension but also with varix of esophagus. Measurement of hpcⅢ and LN are noninvasive test for detection of fibrosis of liver.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1997年第2期177-179,共3页
Journal of China Medical University
