摘要
用核酸与聚阳离子聚甲基丙烯酰氧乙基苄基二甲基氯化铵(PMBDAC)的相互作用导致共振光散射(RLS)增强的现象来测定核酸。考察了pH值、PMBDAC浓度和离子强度对体系共振光散射强度的影响。在优化条件下,建立了用RLS光谱测定微量核酸的新方法。方法的抗干扰能力较强,可允许大部分的常见金属离子、核苷酸、氨基酸、糖、蛋白质等干扰物质的存在。
The determination of nucleic acid is reported based on its interaction with a cationic polymethacryloxylethyl benzyl dimethyl ammonium chloride（PMBDAC） by measurement of resonance light scattering （RLS）. The effect ofpH, concentration of PMBDCA, and ionic strength were investigated. Under the optimum condition, a new method for the determination of micro nucleic acids was established. This method can tolerate the interference of various substances, such as various metal ions, nucleotides, amino acids, and sugars. The synthetic samples weredetermined with satisfactory results.
出处
《胶体与聚合物》
2007年第3期36-38,共3页
Chinese Journal of Colloid & Polymer
基金
湖北省自然科学基金资助项目
关键词
核酸
甲基丙烯酰氧乙基苄基二甲基氯化铵
共振光散射
Nucleic acids
Resonance light scattering
Polymethacryloxylethyl benzyl dimethyl ammonium chloride
