摘要
目的提取并分离纯化不同加工方法处理的梅花鹿鹿茸活性多肽(PAP),并对其免疫功效进行初步研究。方法以不同工艺处理的鹿茸为原料,应用分子排阻层析和离子交换层析提取鹿茸活性多肽,通过淋巴细胞增殖、细胞因子产生等试验,检测PAP对淋巴细胞的免疫功效。结果所提取的梅花鹿鹿茸活性多肽蛋白含量分别为:冻干茸9.20 mg/ml;冷冻鲜茸1.30 mg/ml;热炸茸1.10 mg/ml。鹿茸多肽可以促进小鼠T、B淋巴细胞的增殖,活化巨噬细胞分泌IL-12,对机体有免疫增强作用。结论梅花鹿鹿茸活性多肽对机体的细胞免疫有显著增强作用。
Objective To extract and purify active polypeptides from the pilose antlers treated by various procedures and study their immunological function. Methods Extract active polypeptides from the pilose antlers treated by various procedures by molecular exclusion chromatography and ion exchange chromatography and determine its immunological activity by lymphocyte proliferation test and cytokine production test. Results The protein contents of active polypeptides extracted from freeze-dried,frozen and heat-treated pilose antlers were 9. 2,1.3 and 1.1 mg/ml respectively. The polypeptides promoted the proliferation of murine T and B lymphoeytes, activated macrophages to secrete IL-12 and showed immunopotentiation effect. Concluksion Pilose antler polypeptide enhanced cellular immunity significantly.
出处
《中国生物制品学杂志》
CAS
CSCD
2007年第9期669-673,共5页
Chinese Journal of Biologicals
基金
吉林大学农学部青年基金资助项目(430505010241)
关键词
鹿茸多肽
分子排阻层析
离子交换层析
免疫功效
Pilose antler polypeptide （PAP）
Molecular exclusion chromatography
Ion exchange chromatography
Immunological function
