摘要
大肠杆菌表达系统是基因表达技术中发展最早和目前应用最广的经典表达系统。利用该系统表达重组蛋白具有许多优越性,但其表达效率受诸多因素的影响。综述了国内外利用大肠杆菌表达系统高效表达外源蛋白的策略,主要包括选择合适的启动子、改变信号肽结构、提高 mRNA稳定性、提高翻译效率、表达稀有密码子、降低包涵体形成及蛋白降解,利用融合蛋白与分子伴侣、调控发酵条件实现高密度培养等。
Cloning and expression of recombinant protein in Escherichia coli has been developed for a long time and it is widely used as a classical expression system now. Many advantages of this system have been confirmed, however, disadvantages also exist. The efficiency of expression is affected by many factors. The strategies for high-level expression of recombinant protein in this system were summarized. These strategies mainly include selection of appropriate promoter, optimization of signal peptide, increasing the stability of mRNA and the efficiency of translation, preventing the use of low usage codons, reducing inclusion body formation and degradation of the target protein, using molecular chaperones and fusion protein, development of high-cell-density fermentation, and etc.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第9期103-109,共7页
China Biotechnology
基金
教育部新世纪人才计划资助项目(吴敬)
江苏省自然科学基金资助项目(BK2007019)
关键词
大肠杆菌
基因工程
重组蛋白
克隆
表达
E. coli Gene engineering Recombinant protein Cloning Expression
