摘要
从感病的海南番木瓜叶片中提取总RNA,经RT-PCR和RACE方法分段扩增番木瓜环斑病毒(PRSV)全基因组序列。序列拼接结果显示,PRSV中国海南分离株(HaiNan-P)基因组除3'-末端polyA尾巴外,由10323个核苷酸组成,编码3343个氨基酸。同源性分析表明,HaiNan-P编码的氨基酸序列与GenBank中公布的11株PRSV同源性为89.8%~93.2%,略高于全长核苷酸的82.3%~89.1%。P1蛋白编码区同源性较低,仅为65.4%~80.1%,而HC-Pro、CI及CP同源性相对较高。运用MEGA 3.1软件、NJ法绘制系统进化树,分析结果显示,PRSV分离株类群分化与地理分布有一定的相关性。
Total RNA was purified from infected papaya(Carica papaya L.)leaves in Hainan province,and the full-length sequences of papaya ringspot virus were amplified by RT-PCR and RACE,and its complete genomic sequence was assembled.The linearly assembled single-strand positive sense RNA genome sequence of isolate China-hainan of PRSV was 10323 nucleotides(nts)in length,excluding the 3'-terminal poly(A)tail.And it was conposed of a single open reading frame encoding a polyprotein of 3343 amino acids.The result of homologous analysis showed that the polyprotein of HaiNan-P identity ranged from 89.8%to 93.2%more than the complete nt from 82.3%to89.1%with eleven isolates.The P1 amino acid was the least conserved(with isolates sharing between 65.4%and 80.1%),whereas HC-Pro,CI and CP were the most conserved.Phylogenetic tree were constructed by the neighbor-joining method in MEGA 3.1,which showed that PRSV isolates was obvious relevant to geographical origin.
出处
《广东农业科学》
CAS
CSCD
2007年第9期54-58,共5页
Guangdong Agricultural Sciences
