摘要
为了从富含多酚、多糖及色素的越橘叶片中提取适用于分子生物学研究的高质量基因组DNA。以越橘幼叶为实验材料,比较了CTAB、SDS、高盐低pH值3种提取方法,获得了一种以CTAB法为基础的分离高质量完整DNA的简便、快速方法。用紫外分光光度计、琼脂糖凝胶电泳、RAPD-PCR、酶切等方法对获得的DNA进行了分析,结果表明,快速CTAB法所提取的DNA产量高、质量好,完全能够满足RAPD、PCR等分子生物学实验的要求。
In order to establish a rapid, simple, and effective method of extracting genomic DNA from young leaves of blueberry （Vaccinium spp.）, three conventional methods of CTAB, SDS, and High salty Low pH were compared for the extraction of genomic DNA in blueberry. The results showed that the higher quantity and quality DNA was obtained from the conventional CTAB method. A new method for rapid isolation of genomic DNA in blueberry was established based on the conventional CTAB method. The extracted DNA was assessed by ultraviolet photometer, agarose gel electrophoresis, RAPD-PCR technique, and digestion with restriction endonuclease, respectively, The results showed that the DNA extracted by this rapid method had a high output and purity. The quality of genomic DNA extracted by this rapid method can extensively meet the requirements for many molecular biology experiments. It is a quick and simple way for extracting genomic DNA from blueberry.
出处
《果树学报》
CAS
CSCD
北大核心
2007年第5期714-717,共4页
Journal of Fruit Science
关键词
越橘
基因组DNA
快速提取
Blueberry （Vaccinium spp. ）
Genomic DNA
Rapid isolation
