摘要
目的：研究半夏Pinellia ternata鲜块茎中的生物活性成分，为其质量评价提供科学依据。方法：采用多种柱色谱方法进行分离纯化，根据理化性质和谱学方法鉴定其结构。利用微孔板紫外吸收比色法，体外测定化合物对脂多糖（LPS）刺激的小鼠腹腔巨噬细胞产生肿瘤坏死因子TNF-α的抑制作用。结果：从半夏鲜块茎中分离得到9个化合物，分别鉴定为反式-对-香豆醇（1），3，4-二羟基桂皮醇（2），阿魏酸（3），落叶松脂醇（4），赤式-1-（4-羟基-3-甲氧基苯基）-2-{2，6-二甲氧基-4-[1-（E）-丙稀-3-醇]-苯氧基}-丙烷-1，3-二醇（5），去氢二松柏醇（6），异落叶松脂醇（7），4-羟苯丙烯基-O-β-D-吡喃葡糖苷（8）和松柏苷（9）。化合物1，2，3，8和9在10～mol·L^-1浓度对LPS刺激的小鼠腹腔巨噬细胞产生TNF-α的抑制作用分别为24．1％，57．6％，40．2％，82．7％和62．0％。结论：化合物1，2和4～8系首次从半夏属植物分离得到，化合物9系首次从半夏分离得到。化合物1，2，8和9为苯丙素类化合物，化合物4～7为木脂素类化合物。半夏的抗炎作用可能与化合物1，2，3，8和9有关。
Objective： To study the bioactive constituents of the fresh rhizome of Pinellia ternata, and provide the scientific basis for quality control. Method： Various chromatographic techniques were used to separate and purify the chemical constituents, and their chemical structures were determined on the basis of physical-chemical properties and spectroscopic analysis. The inhibitory effects of the isolated compounds on tumor necrosis factor （TNF）-α production in the peritoneal macrophages of mice stimulated with lipopolysaccharide （LPS） were assayed in vitro by microplate colorimetric method. Result： Nine compounds were isolated and identified as （E）-p-coumaryl alcohol （1）, 3, 4-dihydroxycinnamyl alcohol （2）, ferulic acid （3）, lariciresinol （4）, erythro-guaiacylglycerol-β-O- 4＇-sinapyl ether （5）, dehydrodiconiferyl alcohol （6）, isolariciresinol （7）, sachaliside 1 （8） and coniferin （9）. The inhibitory effect of compounds 1, 2, 3, 8, and 9 were 24. 1%, 57. 6%, 40. 2%, 82. 7%, and 62. 0%, respectively, against the TNF-α production in the peritoneal macrophages of mice stimulated with LPS at a concentration of 10 -5 mol . L^-1 in vitro. Conclusion： The compounds 1, 2 and 4-8 from genera Pinellia Ten. and compound 9 from P. ternata were isolated for the flint time. The compounds 1, 2, 8 and 9 were phenylpropanoids, and 4-7 were lignanoids. The anti-inflammatory effects of the rhizome of P. ternata might relate at the least to compounds 1, 2, 3, 8 and 9.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第17期1755-1759,共5页
China Journal of Chinese Materia Medica
基金
国家"十五"科技攻关项目(99-929-01-02A)
国家计委"现代中药"项目([2002]2323)
关键词
半夏
鲜块茎
化学成分
苯丙素
木脂素
肿瘤坏死因子-Α
Pinellia ternata
fresh rhizome
chemical constituent
phenylpropanoid
lignanoid
TNF-α
