摘要
目的探讨丙泊酚对大鼠肾缺血/再灌注损伤细胞凋亡通路的影响及可能的作用机制。方法建立大鼠肾缺血/再灌注损伤模型,将动物分为对照(Con)组、缺血再灌注(IR)组、缺血再灌注+丙泊酚(IR+P)组。观察损伤肾的形态学改变,用免疫组织化学观察细胞凋亡相关蛋白BCL-2、BAX、caspase3和细胞色素C(cytochrome C)的表达情况。结果光镜可观察到肾缺血/再灌注引起的肾损害,损害程度依次为近曲小管、远曲小管、集合管、肾小球;免疫组化图像分析观察到IR组与Con组相比,BAX、caspase3,细胞色素C表达增加,BCL-2表达未见明显变化;IR+P组与IR组比较,前者BAX、caspase3和细胞色素C表达下降,BCL-2表达增高(P<0·05)。结论丙泊酚对肾缺血/再灌注损伤引起的细胞凋亡可能具有保护作用,可能机制是抑制促凋亡蛋白BAX、caspase3和细胞色素C的表达,对BCL-2的表达无影响,与细胞凋亡的线粒体通路途径有关。
Objective To establish a model of renal ischemic/reperfusion in rats and to observe whether propofol protects kidney from this injury, and to deduce a possible mechanism. Methods Ninty-nine male SD rats with right nephrectomy were randomly divided into three groups according to the different treatments to left kidney： Control group （n =26）, IR group （n =35）, IR ＋Pro group （n =38）. The left kidney was exsected and blood samples were collected 0, 3, 12, 24, 72 h postsurgery respectively. Renal morphology dyed with HE was observed with light microscopy . Immunohistochemistry was used to detect the expression of apoptosis proteins including BCL-2. BAX.caspase 3.cytochrome C. Results Renal ischemia-reperfusion induced tissues injury, the severity of injury sequence was as follows： proximal convoluted tubule, distal convoluted tubule, collecting duct, renal corpuscle; According to the results of IHC, the expression of BAX, caspase 3, and cytochrome C in IR group were more than that in control group, and BCL-2 level was stable. The protective protein （BCL-2） level was higher and the proteins （ BAX, caspase 3, and cytochrome C） inducing apoptosis were lower in IR ＋ P group than that of IR group （P 〈 0. 05 ）. Conclusion Renal ischemia-reperfusion induced tissues injury and the expression changes of apoptosis proteins ; Propofol inhibited the expression of apoptosis proteins such as BAX , caspase 3 , cytochrome C , and induced the action of BCL-2. In a word, propofol perhaps inhibits the apoptosis signaling pathways in mitochondrion and cytoplasm.
出处
《基础医学与临床》
CSCD
北大核心
2007年第8期903-908,共6页
Basic and Clinical Medicine
关键词
丙泊酚
缺血/再灌注
细胞凋亡
propofol
renal ischemia/reperfusion
apoptosis
