摘要
本研究建立了测定食品中苏丹红Ⅰ号含量的间接竞争酶联免疫分析法。首先对苏丹红Ⅰ号分子作了的修饰,再与载体蛋白交联制得免疫原和包被抗原,经动物免疫制得抗苏丹红Ⅰ号的抗体。在包被抗原为100μg/L,抗体为1:100,000倍稀释,标记二抗为1:15000倍稀释的优化条件下,测得检出限为0.12μg/L,IC50值(标准曲线中吸光度抑制至最大吸光度值的50%时所对应的待测物浓度)为0.74μg/L。苏丹红Ⅰ号在番茄酱和辣椒面中的回收率分别为106%和110%。样品仅需甲醇萃取再用缓冲液简单稀释就可以直接进行ELISA测定。
An indirect competitive ELISA for the determination of Sudan I （ 1-phenylazo-2-naphthalenol）, in food samples was developed. Sudan I molecule was modified and coupled to carrier proteins to make the coating antigen and immunogen. The antibodies against Sudan I from rabbits was prepared by a normal immu- nization method. The best combination of the immunoreagents used was 100 μg/L for coating antigen, 1：100 000 dilution for antiserum and 1：15 000 dilution for GaRIgG-hoseradish peroxidase （HRP） conjugate, respectively. LOD （ limit of detection, S/N = 3 ） and IC50 （ the concentration of analyte producing 50% of maximum signal decrease in standard curve） were found to be 0.12 μg/L and 0.74 μg/L, respectively. The recoveries of the detection of tomato sauce and the chilli powder achieved were around 106% and 110% , respectively. After a simple dilution with assay buffer, the sample extract was directly analyzed by the developed ELISA.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1168-1170,共3页
Chinese Journal of Analytical Chemistry
基金
教育部留学回国人员科研启动基金支持项目(No2006331-11-3)
