摘要
本研究１．建立了检测ＰＣＤ的石蜡切片原位末端标记（ＩＳＥＬ）法，在石蜡切片原位用免疫组化法标记核ＤＮＡ，使ＰＣＤ细胞呈黄色或棕黄色的阳性着染，在普通光学显微镜下即可鉴别。本实验用加热和蛋白酶Ｋ同时处理背景，使非特异性染色几乎不存在；使用过氧化物酶—ＤＡＢ显色系统缩短时间２小时，提高了筛检速度。２．应用ＩＳＥＬ法研究外来激素对大鼠睾丸细胞的影响，结果发现ＧｎＲＨ－Ａ是一个促凋原，具有明显的生殖毒性。
In order to evaluate the reproductive toxicity of environmental hormones and explore the mechanism of male infertility,the present experiment used in situ ending labeling (ISEL) assay to analyse the effects of environmental chemicals on apoptosis.Firstly established the method of ISEL,and secondly used the ISEL to measure the reproduction toxicity of GnRH-A.ISEL has the advantages of high specifity and sensitivity,and good repetition.In situ analysis of developing testis demonstrated that spermatocytes and spermatogonia constituted the major PCD cell types.That PCD rate in GnRH-A group was apparently higher than that of the control group showed that GnRH-A is an apogen exerting reproduction toxicity.
出处
《癌变·畸变·突变》
CAS
CSCD
1997年第1期23-28,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
PCD检测
激素
睾丸细胞
细胞凋亡
原位末端标记
Programmed cell death(PCD)
In situ end labeling(ISEL)
Testis germ cell
Gonadotroping releasing hormone analogue(GnRH-A)
