摘要
目的了解氟对大鼠雄性生殖系统的危害。方法30只雄性SD大鼠随机分为对照组、高剂量染毒组、低剂量染毒组，分别腹腔注射蒸馏水及20、10mg／kg NaF溶液，39d后观察大鼠体重、精子数目变化，采用原位杂交法检测睾丸生殖细胞端粒酶逆转录酶（TERT）表达，用免疫组化法检测睾丸生殖细胞中增殖细胞核抗原（PCNA）的表达。结果低、高剂量染毒组体重分别为（273．39±20．68）、（240．00±21．39）g，低于对照组，差异有统计学意义（P〈0．05）；TERT表达率分别为（13．89±4．86）％、（6．33±4．42）％，PCNA表达率分别为（0．71±0．05）％、（0．60±0．08）％，均低于对照组，差异有统计学意义（P〈0．05）；精子数目分别为（18．31±1．20）×10^10／L、（9．17±1．38）×10^10／L，低于对照组，差异有统计学意义（P〈0．05）。结论氟可降低生殖细胞TERT、PCNA的表达，对雄性生殖系统造成损害。
Objective To study the damages of fluoride on the male reproductive system in rat testes. Methods A total of 30 male SD rats were randomly divided into control group,high,low dose fluorine treated groups, which were given normal saline, 20 mg/kg sodium fluoride, and 10 mg/kg sodium fluoride respectively. After 39 days the change of the weight of rats and the number of sperms were observed. The change of telomerase reverse transcriptase（TERT） and proliferating cell nuclear antigen（PCNA） were observed by using in situ hybridization and radioimmunoassay respectively. Results The weight was （273.39±20.68）, （240.00±21.39）g in NaF treated groups,which was lower than that in control group（P〈0.05）; The rate of TERT expression in germ cells of testes in NaF treated groups was（ 13.89±4.86）% and （6.33±4.42）% respectively, which was significantly lower than that in control group （P〈0.05）.The rate of PCNA expression in germ cells of tests in NaF treated groups was （0.71±0.05）%,（0.60±0.08）% respectively, which also was significantly lower than that in control greup（P〈0.05）. The number of sperms was （18.31±1.20）×10^10/L, （9.17±1.38）×10^10/L, which was lower than that in control group（P〈0.05）. Conclusion Fluorine possibly damages the male reproductive system by reducing the expression of TERT and PCNA.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第2期96-99,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
