摘要
利用子叶下胚轴为外植体,通过农杆菌Ti质粒介导遗传转化途径,建立了表达白羽扇豆酸性磷酸酶基因(APase)的白三叶草转基因系。PCR检测发现,APase基因已整合到转基因植株的基因组中。Northern印迹分析表明,APase基因在部分转基因系中高水平表达。此外,在异源表达APase的白三叶草中,位于APase上游的Patatin信号肽具有将翻译产物引导至细胞间隙和根际的能力。在以植酸盐为唯一磷素供源的条件下,与转化空载体的对照植株相比,高水平表达APase的白三叶草转基因系植株的磷素累积量、鲜重和干重显著增加。结果表明,异源表达白羽扇豆APase基因能明显增强白三叶草吸收有机态磷的能力。
Transgenic Trifolium repens lines ectopic expressing the APase gene of Lupinus albus were established from T. repens cotyledon explants derived from Agrobacteriurn tumefaciens-mediated methods. PCR analysis indicated that the APase gene was integrated into the genome of transgenic T. repens lines and Northern blot results showed that the APase gene in some transgenic T. repens lines was expressed at high levels. It was also found that the translated APase in transgenic T. repens lines directed by a patatin signal peptide could be secreted to the apoplast and the rizhosphere. Under the growth conditions in which the phytate was the sole source of phosphate, the accumulated amount of P, fresh weight and dry weight of transgenic plants were all significantly higher than those of the empty vector transformed control plants （CK）. Therefore, the ectopic expression of the L. albus APase can significantly increase the ability of T. repens to utilize organic phosphate.
出处
《草业学报》
CSCD
2007年第4期69-75,共7页
Acta Prataculturae Sinica
基金
国家粮食丰产科技工程项目(2004BA520A07-04)
河北省自然科学基金(2006000434)资助
关键词
三叶草
遗传转化
白羽扇豆APase基因
磷素吸收
Trifolium repens
genetic transformation
APase gene from Lupinus albus
phosphorus uptake
