摘要
冷凝蛋白（ｃｏｌｄｉｎｓｏｌｕｂｌｅｐｒｏｔｅｉｎ，ＣＩＰ）是机体血浆经低温致冷后形成的大分子冷不溶性蛋白，它在机体组织冷损伤起着一定的作用。ＣＩＰ的形成与纤维粘连蛋白（ｆｉｂｒｏｎｅｃｔｉｎ，Ｆｎ）有关。本文观察到猪血浆Ｆｎ的胰蛋白酶酶切片段混合物（Ｆｎｆｒａｇｍｅｎｔｓ，Ｆｎｆ）在５×１０－６ｍｏｌ／Ｌ浓度时，能明显抑制新鲜大白鼠血浆中ＣＩＰ的形成，血浆中ＣＩＰ的形成量（７９±４６μｇ／ｍｌ）仅为对照血浆组（７８１±２６０μｇ／ｍｌ）的１０％，推测Ｆｎ分子上可能有相应的结构参与了ＣＩＰ的形成。
CIP(cold insoluble protein) is a cold insoluble protein with large molecular weight.In the present study, fibronectin was separated and purified from fresh pig plasma with affinity chromatography, and fibronectin fragments(Fnf) mixture(Wm=60kD) was obtained by the method of fixed trypsin. While Fnf was added into fresh rat plasma up to 5×10 -6 mol/L, CIP formation(79±46μg/ml)was inhibited markedly, only 10%(15℃) as much as that of the control group(781±260μg/ml). The results suggested that some part of the structure(s) of Fn molecule might take part in the plasma CIP formation.
出处
《解放军预防医学杂志》
北大核心
1997年第2期93-96,共4页
Journal of Preventive Medicine of Chinese People's Liberation Army
关键词
冷凝蛋白
纤维粘连蛋白
片段
cold insoluble protein (CIP)
fibronectin(Fn)
fibronectin fragments(Fnf)
