摘要
目的应用荧光光谱法测定小鼠血浆中异丙酚的浓度.方法在含不同量异丙酚的小鼠血浆中加入磷酸缓冲液及环己烷/正丁醇,振荡、离心后取上清液测荧光强度(发射波长299 nm,激发波长270 nm).结果异丙酚投入量(0.1～10 mg·L-1)与荧光强度(INT)间有良好的线性关系(r=0.999 6).结论该方法灵敏度高,线性范围大,操作简便,专一性好,可用于临床异丙酚血药浓度的监测.
Aim To determine the concentration of propofol in blood plasma of mice with spectrofluorometry. Methods Buffer solution of phosphoric acid and cyclohexane-tertbutanol were added to blood plasma with different concentration of propofol, which were vibrated and centrifugalized. After that, the upper-clear solution was taken to determine the fluorescent intensity （excitation wave 270 nm, emission wave 299 nm）. Results The experimental data exhibited a good linear relationship between the logarithms of the fluorescent intensity and the amount of propofd added to the blank plasma in the scale of 0.1-10 mg·L^-1 （r=0. 9996）. Conclusion This method is good at sensitivity. Linearity range is simple. The convenient, economical ,accurate and specific procedure can be applied to determine the concentration of propofol in blood clinically.
出处
《安徽医药》
CAS
2005年第8期586-587,共2页
Anhui Medical and Pharmaceutical Journal
