摘要
目的:探讨通心络对兔模型颈动脉粥样硬化性斑块的三磷酸腺苷结合盒运转体Al (ABCAl)、视黄酸X受体(RXRα)信使核糖核酸(mRNA)表达的影响及机制。方法:32只新西兰大白兔分为4组,其中空白对照组8只(A组),余24只兔于右侧颈动脉放置改良的硅橡胶圈,加1%高胆固醇喂养的方法建立粥样硬化性颈动脉狭窄动物模型。颈动脉狭窄模型而无药物干预的对照组8只(B组);辛伐他丁治疗组8只,给予辛伐他丁5 mg/kg,每天1次(C组);通心络治疗组8只,给予通心络1 g/kg,每日1次(D组)。药物干预前后分别检测兔模型静脉血的血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL)及高密度脂蛋白胆固醇(HDL)水平,两种药物干预2周后处死动物取右侧颈动脉狭窄段及对侧相应段血管,以Western Blot法测定其ABCAl、RXRα蛋白质表达量。结果:给予通心络和辛伐他丁干预兔模型组(C组和D组)ABCAl、RXRα蛋白质表达水平均增高,血脂水平下降。结论:通心络可使模型兔颈动脉粥样硬化斑块的ABCAl mRNA、RXRαmRNA表达水平和其蛋白质表达水平上调,可能有益于抗动脉粥样硬化斑块形成作用。
Objective： To investigate the effects of TONGXINLUO capsule on the expressions of ATP-binding cassette transporter A1（ABCA1） in the rabbits with carotid artery atherosclerotic plaque, hereby to explore possible mechanism through which ABCA1 affects the formation of atherosclerosis. Methods： Improved silicon callar was positioned at the carotid artery of male New Zealand white rabbits and they were fed with hypsocholesterol diet for 2 weeks to establish the experimental carotid artery stenosis models. Twenty-four rabbits with carotid artery atherosclerotic（CAS） plaque were randomly divide dinto three groups,which were subsequently treated with TONGXINLUO capsule（1g/kg ·d^-1 ）and simvastin（5mg/kg · d^-1 ）respectively for 2 weeks. The rabbit carotid artery atherosclerotic plaque was excised 2 weeks after treatment, the protein levels of ABCA1 and RXRα were detected by Western blot respectively. Results： The protein expression levels of ABCA1 and RXRα all increased after treatments with TONGXINLUO capsules and simvastin. Conclusions： TONGXINLUO capsules may offer protective effects against CAS plaque.
出处
《临床神经电生理学杂志》
2007年第4期195-198,共4页
Journal of Clinical Electroneurophysiology
