摘要
目的:观察葛根素对去卵巢骨质疏松性骨折大鼠胰岛素样生长因子1表达的影响。方法:实验于2005-02/2006-01在解放军第四五四医院骨科和南京医学院完成。实验材料:葛根素购自中国生物药品鉴定所。SD雌性大鼠40只,8月龄,体质量(280±19)g,由南京医学院实验动物中心提供。实验方法:将大鼠用4%戊巴比妥钠40mg/kg腹腔内麻醉,在无菌条件下行双侧卵巢摘除术,术后继续饲养2个月,借助骨折器建立股骨闭合性骨折模型。按随机排列表法分为对照组和治疗组,每组20只。治疗组术后给予灌胃20mg/(kg·d)剂量的葛根素,对照组给予2mL生理盐水处理。于骨折后第2周利用Western-blot和RT-PCR方法检测胰岛素样生长因子1蛋白与胰岛素生长因子1mRNA表达,观察葛根素对胰岛素生长因子1表达的影响。结果:40只大鼠全部进入结果分析。①Western-blot检测胰岛素生长因子1蛋白:与对照组相比,治疗组胰岛素生长因子1蛋白表达明显升高(1.40±0.09,3.2±0.31,P<0.05)。②RT-PCR分析胰岛素生长因子1mRNA表达:与对照组相比,治疗组胰岛素生长因子1mRNA表达明显升高(12.3±2.09,23.1±1.32,P<0.05)。结论:葛根素可以通过调节胰岛素生长因子1的表达,从而可能加快去卵巢大鼠骨质疏松性骨折的愈合。
AIM： To observe the effect of puerarin on the expression of insulin-like growth factor-1 （IGF-1） in ovariectomized rats with osteoporotic fracture.
METHODS： The experiment was conducted in the Department of Orthopedics, the 454 Hospital of Chinese PLA and Nanjing Medical College from February 2005 to January 2006. Forty female SD rats of 8 months old and （280±19） g were anesthetized by intraperitoneal injection of 4% somnopentyl （40 mg/kg） to remove the ovaries. After feeding for 2 months, the models of closed fracture of femur were established assisted by fracture tool. Then the rats were randomly divided into control group and treatment group with 20 animals in each group. The treatment group was given the puererin at dose of 20 mg/kg daily, while the control group was given 2 mL normal saline. IGF-1 protein and mRNA expressions were detected by Westem blot and RT-PCR to observe the effect of puerarin.
RESULTS： Totally 40 rats entered the result analysis without loss. ①Westem-blot results showed that compared with the control group, the expression of IGF-1 protein of the treatment group was significantly increased （1.40±0.09, 3.2±0.31, P 〈 0.05）. ②RT-PCR analysis suggested that compared with the control group, the expression of IGF-1 mRNA of the treatment group was significantly increased （12.3±2.09, 23.1±1.32, P 〈 0.05）.
CONCLUSION： Puerarin could accelerate the bone fracture healing of ovariectomized rats by regulating the expression of IGF-1.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第32期6406-6409,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
