摘要
由于受到发酵罐溶氧条件的限制,在高浓度生物素的谷氨酸发酵中往往出现产酸与糖酸转化率不协调的现象,针对这一现象,研究了二次接种叠加生物素的谷氨酸发酵工艺。在试验所用的发酵罐中,采用8.0μg/L生物素浓度的培养基作为发酵基础培养,经过一段时间发酵后,接入第二次种子液以及3.0μg/L(发酵液初始体积)的生物素量,通过适当的发酵控制,产酸水平达到139.6g/L,糖酸转化率高达62.80%,单罐谷氨酸产量比一次接种添加8.0μg/L生物素的发酵工艺提高了15.78%。
Inconsistence of glutamic acid production with yield of glutamic acid to glucose often occurs in high biotin fermentation process because of limited fermentor dissolved oxygen coefficient. Glutamic acid fermentation process with two inoculations and additions of biotin were investigated to solve the inconsistence. Second inoculation and 3μg/L biotin were added after cultivation for some time with 8μg/L biotin in the fermentor. Glutamic acid production reached 139.6g/L and yield of glutamic acid to glucose reached 62.80%. The single fermentor glutamic acid outcome increased by 15.78% than that of single inoculation and addition of 8.0μg/L biotin.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第7期1-5,共5页
Food and Fermentation Industries
关键词
二次接种
叠加
生物素
谷氨酸
溶氧
two inoculations, repeated addition, biotin, glutamic acid, dissolved oxygen
